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Progesterone Receptor in the Chick Bursa of Fabricius: Characterization and Immunohistochemical Localization

TIMO YLIKOMI, JEAN-MARIE GASC^*,, JORMA ISOLA, ETIENNE-EMILE BAULIEU and PENTTI TUOHIMAA

Department of Biomedical Sciences, University of Tampere SF-33101 Tampere, Finland

Address all correspondence and requests for reprints to: Dr. Pentti Tuohimaa, Department of Biomedical Sciences, University of Tampere, Box 607, Tampere, SF33101 Finland.

Abstract

A high affinity progesterone-binding site was studied in the chick bursa of Fabricius. The dissociation constant for progesterone was 1.4 nM, and the concentration of progesterone-binding sites increased with estradiol treatment. In estradiol-treated bursas, the receptor concentration was about 240 fmol/mg protein. The binding site was specific for progestins, with the following order of affinities: ORG 2058 > progesterone > promegestone. Androgens, dexamethasone, and estradiol were weak competitors for progesterone binding in the bursa cytosols from estradiol-treated chicks. Immunoglobulin G fraction of antiserum (immunoglobulin G-RB) raised in rabbit against the B-subunit of chick oviduct progesterone receptor (PR) was used for an immunohistochemical study. The PR was found only in the interfollicular cells, which were most probably nonlymphoid cells. Staining was localized exclusively in the elongated nuclei of these cells. No staining was seen in the bursal epithelium or inside the lymphoid follicles.

The results indicate that the interfollicular cells of the bursa contain specific PRs which are under estrogen regulation as in the oviduct. Thus, these cells might be under direct progesterone regulation. (Endocrinology 117: 155–160, 1985)

Footnotes

^* On leave from the Institut d’Embryologie du CNRS and du College de France, Nogent sur Marne, France.

Present address: INSERM U33, Lab Hormones, 94270 Bicetre, France.

Received September 9, 1984.

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