Endocrinology, Vol 117, 790-792, Copyright © 1985 by Endocrine Society

ARTICLES

A simple and rapid method using polyethylenimine-treated filters for assay of solubilized LH/hCG receptors

PC Roche, ER Bergert and RJ Ryan

Filtration through polyethylenimine (PEI)-treated membrane filters efficiently separates free radiolabeled human (h) LH or hCG from solubilized receptor-hormone complexes. Solubilized LH/hCG receptors of bovine, porcine, and rat origin are suitable for measurement by this method. Specific binding assayed by the PEI-filter technique was 106- 138% of specific binding assayed by double polyethylene glycol precipitation, demonstrating that the method gave quantitative recovery of bound hormone. Nonspecific binding was consistently lower with PEI- treated filters. Intra- and interassay variability for the two different methods was similar. These results indicate that filtration through PEI-treated membrane filters is a simple and rapid method for assay of solubilized LH/hCG receptors.

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