help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ryan, M. P.
Right arrow Articles by Duckworth, W. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ryan, M. P.
Right arrow Articles by Duckworth, W. C.

Endocrinology, Vol 117, 1693-1698, Copyright © 1985 by Endocrine Society

ARTICLES

The calcium dependence of insulin degradation by rat skeletal muscle

MP Ryan, JD Gifford, SS Solomon and WC Duckworth

The effect of calcium on insulin degradation by intact or homogenized skeletal muscle, by skeletal muscle cytosol, and by partially purified skeletal muscle insulin-degrading protease activity was examined. After a 15-min lag phase, intact soleus muscles degraded [125I]insulin to trichloroacetic acid-soluble products in a time-dependent fashion. Degradation was accelerated by the addition of calcium (greater than or equal to 1 mM), such that maximal stimulation (2-fold) was obtained with 10 or 25 mM calcium. Calcium stimulated insulin degradation by skeletal muscle homogenate and by cytosol in a nearly identical manner. Furthermore, after inactivation of the purified skeletal muscle, insulin-degrading protease by dialysis against EDTA, this enzyme was reactivated fully (greater than 80%) by a 100 microM concentration of free Ca2+. These observations identify a previously unrecognized but important influence of calcium on cellular insulin handling and provide further evidence for a major role of the calcium-activated enzyme, insulin protease, in cellular insulin degradation.


This article has been cited by other articles:


Home page
 J. Am. Coll. Nutr.Home page
M. E. Valera Mora, A. Scarfone, M. Calvani, A. V. Greco, and G. Mingrone
Insulin Clearance in Obesity
J. Am. Coll. Nutr., December 1, 2003; 22(6): 487 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
W. C. Duckworth, R. G. Bennett, and F. G. Hamel
Insulin Degradation: Progress and Potential
Endocr. Rev., October 1, 1998; 19(5): 608 - 624.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
F. G. Hamel, R. G. Bennett, and W. C. Duckworth
Regulation of Multicatalytic Enzyme Activity by Insulin and the Insulin-Degrading Enzyme
Endocrinology, October 1, 1998; 139(10): 4061 - 4066.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1985 by The Endocrine Society