Endocrinology, Vol 118, 138-142, Copyright © 1986 by Endocrine Society

ARTICLES

Induction of epidermal growth factor-related polypeptides by 17 beta- estradiol in MCF-7 human breast cancer cells

RB Dickson, KK Huff, EM Spencer and ME Lippman

MCF-7 human breast cancer cells release polypeptides related to insulin- like growth factor-I (IGF-I) and epidermal growth factor (EGF) into serum-free culture medium (CM). Treatment of MCF-7 cells with 17 beta- estradiol, which is required in vivo for MCF-7 tumor growth in the nude mouse and stimulates the MCF-7 growth rate in vitro, resulted in selectively enhanced growth factor activities in CM. Autostimulatory growth-promoting activity was elevated at least 2-fold, and EGF-like polypeptides were elevated 5-fold but IGF-I immunoreactivity was not elevated. Several species of estrogen-induced receptor-reactive EGF- like polypeptides, suggestive of high molecular weight transforming growth factor alpha, were detected after gel exclusion chromatography of CM extracted with 1 M acetic acid. A 30,000 mol wt peak of EGF receptor competing activity comigrated with a peak of autostimulatory and fibroblast-transforming activity. It is possible that estradiol stimulation of MCF-7 growth and/or tumor formation may depend on induction of EGF-related polypeptide growth factors. EGF-I- and EGF- related polypeptides may act together as autocrine or paracrine growth factors in breast cancer.

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