This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by DICKSON, R. B. Articles by LIPPMAN, M. E. Search for Related Content PubMed PubMed Citation Articles by DICKSON, R. B. Articles by LIPPMAN, M. E.

Induction of Epidermal Growth Factor-Related Polypeptides by 17β-Estradiol in MCF-7 Human Breast Cancer Cells

Medical Breast Cancer Section, Medicine Branch, National Cancer Institute, National Institutes of Health Bethesda, Maryland 20205;
Children’s Hospital of San Francisco San Francisco, California 94118

Address requests for reprints to: Dr. Marc E. Lippman, Medical Breast Cancer Section, National Cancer Institute, National Institutes of Health, Building 10, Room 12N226, Bethesda, Maryland 20205.

Abstract

MCF-7 human breast cancer cells release polypeptides related to insulin-like growth factor-I (IGF-I) and epidermal growth factor (EGF) into serum-free culture medium (CM). Treatment of MCF-7 cells with 17β-estradiol, which is required in vivo for MCF-7 tumor growth in the nude mouse and stimulates the MCF-7 growth rate in vitro, resulted in selectively enhanced growth factor activities in CM. Autostimulatory growth-promoting activity was elevated at least 2-fold, and EGFlike polypeptides were elevated 5-fold but IGF-I immunoreactivity was not elevated. Several species of estrogen-induced receptor- reactive EGF-like polypeptides, suggestive of high molecular weight transforming growth factor, were detected after gel exclusion chromatography of CM extracted with 1 M acetic acid. A 30,000 mol wt peak of EGF receptor competing activity comigrated with a peak of autostimulatory and fibroblast-transforming activity. It is possible that estradiol stimulation of MCF- 7 growth and/or tumor formation may depend on induction of EGF-related polypeptide growth factors. EGF-I- and EGF-related polypeptides may act together as autocrine or paracrine growth factors in breast cancer. (Endocrinology 118: 138–142, 1985)

Received July 18, 1985.

This article has been cited by other articles:

				L. Ciarloni, S. Mallepell, and C. Brisken Amphiregulin is an essential mediator of estrogen receptor {alpha} function in mammary gland development PNAS, March 27, 2007; 104(13): 5455 - 5460. [Abstract] [Full Text] [PDF]

				D. Bhandare, R. Nayar, M. Bryk, N. Hou, R. Cohn, N. Golewale, N. P. Parker, R. T. Chatterton, A. Rademaker, and S. A. Khan Endocrine Biomarkers in Ductal Lavage Samples from Women at High Risk for Breast Cancer Cancer Epidemiol. Biomarkers Prev., November 1, 2005; 14(11): 2620 - 2627. [Abstract] [Full Text] [PDF]

				M. H. Herynk and S. A. W. Fuqua Estrogen Receptor Mutations in Human Disease Endocr. Rev., December 1, 2004; 25(6): 869 - 898. [Abstract] [Full Text] [PDF]

				E. R. Levin Bidirectional Signaling between the Estrogen Receptor and the Epidermal Growth Factor Receptor Mol. Endocrinol., March 1, 2003; 17(3): 309 - 317. [Abstract] [Full Text] [PDF]

				J. Russo, Y.-F. Hu, X. Yang, and I. H. Russo Chapter 1: Developmental, Cellular, and Molecular Basis of Human Breast Cancer J Natl Cancer Inst Monographs, July 1, 2000; 2000(27): 17 - 37. [Abstract] [Full Text] [PDF]

				A. T. Baron, J. M. Lafky, C. H. Boardman, S. Balasubramaniam, V. J. Suman, K. C. Podratz, and N. J. Maihle Serum sErbB1 and Epidermal Growth Factor Levels As Tumor Biomarkers in Women with Stage III or IV Epithelial Ovarian Cancer Cancer Epidemiol. Biomarkers Prev., February 1, 1999; 8(2): 129 - 137. [Abstract] [Full Text]

				Y. Watabe, H. Kuramochi, Y. Furuya, N. Inagaki, S. Seino, S. Kimura, and J. Shimazaki Identification of Histone H2A.X as a Growth Factor Secreted by an Androgen-independent Subline of Mouse Mammary Carcinoma Cells J. Biol. Chem., October 11, 1996; 271(41): 25126 - 25130. [Abstract] [Full Text] [PDF]

				J. DePasquale, W. Samsonoff, and J. Gierthy 17-beta-Estradiol induced alterations of cell-matrix and intercellular adhesions in a human mammary carcinoma cell line J. Cell Sci., January 5, 1994; 107(5): 1241 - 1254. [Abstract] [PDF]

				R. Dickson, M. McManaway, and M. Lippman Estrogen-induced factors of breast cancer cells partially replace estrogen to promote tumor growth Science, June 20, 1986; 232(4757): 1540 - 1543. [Abstract] [PDF]