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Steroid-Binding Proteins in Primate Plasma^*

WILLIAM ROSNER, MICHEL M. PUGEAT, GEORGE P. CHROUSOS and M. SAEED KHAN

St. Luke’s-Roosevelt Hospital Center and Columbia University College of Physicians and Surgeons New York, New York 10019
The Developmental Endocrinology Branch, National Institute of Child Health and Human Development Bethesda, Maryland 20205

Address requests for reprints to: William Rosner, M.D., The Roosevelt Hospital, 428 West 59th Street, New York, New York 10019.

Abstract

We used immunological techniques to compare the serum corticosteroid-binding globulins (CBG) and testosterone-estradiol-binding globulins (TeBG) of Old World primates (man, chimpanzee, cynomologus, and rhesus), New World monkeys (squirrel and owl), and prosimians (galago and lemur).

Four different antihuman TeBG antisera could not differentiate human and chimpanzee TeBG and recognized the galago and lemur TeBG as similar as well as the rhesus and cynomologus TeBG, as similar. Western blots of serum subjected to sodium dodecyl sulfate gel electrophoresis, with detection by an anti-TeBG antiserum, showed similar patterns of distribution of the two molecular species of TeBG for all of the New World primates and the owl monkey. The abundance of the two TeBG species was reversed in squirrel monkey serum, while lemur and galago displayed only a single band.

Four different antihuman CBG antisera grouped together the CBGs of human and chimpanzee, rhesus and cynomologus, and lemur and galago. The squirrel monkey has a CBG with a markedly decreased affinity for cortisol; all four antisera perceived its CBG as much more immunologically distant from the human protein than that of the owl monkey. Indeed, three of the four antisera grouped squirrel monkey CBG with that of the prosimians, while one antiserum saw squirrel monkey CBG as even more distant from the human protein than the CBG of the primitive primates, the prosimians. (Endocrinology 118: 513–517, 1986)

Footnotes

^* This work was supported in part by USPHS Grant AM-28562.

Present address: INSERM U.34, Hopital Debrousse, 69322 Lyon, France.

Received July 30, 1985.