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Estrogen Receptors in Cultured Rat Uterine Cells: Induction of Progesterone Receptors in the Absence of Estrogen Receptor Processing^*

Departments of Biochemistry and Animal Sciences, University of Wisconsin Madison, Wisconsin 53706

Address requests for reprints to: Dr. Jack Gorski, Department of Biochemistry, University of Wisconsin-Madison, 420 Henry Mall, Madison, Wisconsin 53706.

Abstract

When cultured rat uterine cells were treated for up to 6 h with 5 nM 17β-estradiol, no decrease in the [³H] estradiol-binding capacity of the cells was observed (i.e. no processing). This was true whether the cells were treated directly with 5 nM [³H]estradiol or with 5 nM unlabeled 17β-estradiol followed by homogenization and exchange with [³H]estradiol in vitro. In additional experiments, intact cells were treated with medium containing 5 nM [³H]estradiol for 30 min, and then that medium was removed and replaced with medium containing 5 nM unlabeled 17β-estradiol. Receptor-bound estradiol in intact cells was totally exchangeable with estradiol in the culture medium (t 90 min). Six-hour treatment of cells with 5 nM 17β-estradiol led to a 50% increase in the [³H]progesterone-binding capacity of the cells, while no loss of estrogen-binding capacity occurred. These results indicate that progesterone receptors can be induced by estrogen in the rat uterus in the absence of estrogen receptor processing. (Endocrinology 118: 603–608, 1986)

Footnotes

^* This work was supported in part by the College of Agricultural and Life Sciences, University of Wisconsin, Madison; NIH Grant HD-08192, and NIH Training Grant 5T32-GM07215 (to J.K.). This is paper 830 from the Department of Meat and Animal Science.

Received October 24, 1984.