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Gonadotropin Depression of Adenosine Triphosphate Levels and Interaction with Adenosine in Rat Granulosa Cells^*

Department of Physiology, University of Göteborg Göteborg, Sweden

Address all correspondence and requests for reprints to: Håkan Billig, Department of Physiology, University of Göteborg, Box 33031, S-400 33 Göteborg, Sweden.

Abstract

Cellular ATP levels were measured with the luceferin-luciferase enzyme method in incubated preovulatory granulosa cells in vitro from PMSG-treated immature rats. The ATP levels were depressed by both FSH and LH, FSH being the more effective. Adenosine enhanced the ATP levels about 3-fold, but the depressive effects of gonadotropins could not be overcome by the addition of adenosine. Uptake of adenosine in granulosa cells followed Michaelis-Menten kinetics, with a K_m of 15.9 ± 3.6 µM and a maximum velocity of 1.6 ± 0.1 pmol/min · 10⁵ cells. The half-time for uptake of adenosine was about 40 min. The maximal uptake of adenosine was lowered from 48 ± 5 to 30 ± 1 pmol/10⁵ cells by FSH treatment of the cells. The basal secretions of cAMP and progesterone from the granulosa cells were slightly but significantly enhanced by adenosine alone. Adenosine markedly enhanced FSH-stimulated cAMP secretion, but not progesterone secretion. A nonmetabolizable adenosine analog, 2-chloro-adenosine, did not affect the ATP levels or the secretion of cAMP from granulosa cells. This study confirms previous observations that adenosine can increase ATP levels and amplify the response to gonadotropins in gonadal cells. A novel finding is that the levels of ATP in granulosa cells are markedly depressed by gonadotropins. It is speculated that this depression of ATP may be a factor in the metabolic control of granulosa cells. (Endocrinology 118: 645–652, 1986)

Footnotes

^* This work was supported by grants from the Swedish Medical Research Council (no. 27, 5650 and 7314), the Swedish Society for Medical Research, Göteborg Medical Society, and the Faculty of Medicine, University of Göteborg. A preliminary report of this work was presented at the Seventh International Congress of Endocrinology, Quebec City, Canada, 1984 (Abstract 167).

Received June 11, 1985.

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