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Influence of Lactation upon Pancreatic Islet Function^*

CORINNE J. HUBINONT, SIMON P. DUFRANE, PILAR GARCIA-MORALES, ISABEL VALVERDE, ABDULLAH SENER and WILLY J. MALAISSE

Laboratory of Experimental Medicine, Brussels Free University Brussels, Belgium
Fundacion Jimenez Diaz, Universidad Autonoma de Madrid, Madrid, Spain

Address requests for reprints to: W. J. Malaisse, M.D., Laboratory of Experimental Medicine, Brussels Free University, Boulevard de Waterloo 115, Brussels B-1000, Belgium.

Abstract

The activity of adenylate cyclase, its reponsiveness to NaF and forskolin, the activity of the protein kinases A and C, and the oxidation of exogenous D-glucose, L-leucine, and L-glutamine were all higher in pancreatic islets removed from lactating, as distinct from nonlactating, rats. Yet, the release of insulin evoked by D-glucose or the association of L-leucine and L-glutamine was lower in the islets obtained from lactating animals. The lactation-induced decrease in secretory activity was not attributable to a change in the insulin content of the islets, was not corrected by exposure of the islets to theophylline or forskolin, and was also observed in response to stimulation by Ba²⁺. The rapidly exchangeable islet Ca pool, as estimated from the basal value for ⁴⁵Ca net uptake, was severely decreased in lactation. Moreover, a hypoglycemic sulfonylurea, which stimulated islet ⁴⁵Ca net uptake much more markedly in lactating than nonlactating animals, provoked, in association with 12-O-tetradecanoylphorbol-13-acetate, a greater insulin output in lactating than nonlactating rats. It is speculated that the decreased secretory activity in islets removed from lactating rats may be accounted for, in part at least, by a decreased Ca content of the islet cells. (Endocrinology 118: 687–694, 1986)

Footnotes

^* This work was supported by grants from the Belgian Foundation for Scientific Medical Research, Belgian Ministry of Scientific Policy, and Spanish National Health Institute.

Research Fellow of the Erasme Foundation (Brussels, Belgium).

Received August 26, 1985.

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