Endocrinology, Vol 118, 1072-1080, Copyright © 1986 by Endocrine Society

ARTICLES

Processing of insulin-like growth factors I and II by capillary and large vessel endothelial cells

RS Bar, M Boes and M Yorek

Cultured endothelial cells from bovine capillaries and pulmonary arteries were incubated with highly purified [125I]insulin-like growth factor I ([125I]IGF-I), [125I]IGF-II, or [125I]insulin for periods up to 120 min, the cells were washed, and the cell-bound radioactivity was allowed to dissociate from the cells into fresh incubation medium. For insulin, 85-95% of the 125I dissociated from cells in 15 min, with 75% dissociating by 5 min. The 125I material released into the medium during the first 5 min of dissociation was entirely intact insulin, while the material released in the next 10 min was 80% intact insulin. For the [125I]IGFs, several differences were observed. First, after 5 min of dissociation, only 30-45% of the cell-bound 125I was released into the medium. For IGF-I, this rapidly dissociating material was entirely intact peptide, whereas for IGF-II, up to 55% of the dissociated radioactivity was degraded peptide. Second, during the next 10 min of dissociation, an additional 20% of the 125I was released from the cells. For IGF-I, this was 85% intact peptide; however, for IGF-II, this dissociated fraction contained as little as 25% intact peptide. Third, for both IGFs, after 15 min of dissociation, 40-65% of the initial cell-associated 125I remained within the endothelial cells; after the 15-min dissociation period, 95% of the remaining internalized 125I was intact IGF-I, whereas for IGF-II, the internalized 125I was 90% intact IGF-II after short periods of association (less than 15 min) and progressively decreased to 65% intact peptide after 60 min of association. We conclude that in addition to having separate surface receptors for insulin, IGF-I, and IGF-II, endothelial cells also process each hormone by distinct pathways.

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