This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by TANAKA, Y. Articles by IMURA, H. Search for Related Content PubMed PubMed Citation Articles by TANAKA, Y. Articles by IMURA, H.

Effect of 1,25-Dihydroxyvitamin D₃ on Insulin Secretion: Direct or Mediated?

Department of Pediatrics, Osaka University School of Medicine Fukushima-ku, Osaka 553, Japan
Division of Metabolism and Clinical Nutrition, Kyoto University School of Medicine Sakyo-ku, Kyoto 606, Japan
Second Division, Department of Internal Medicine, Kyoto University School of Medicine Sakyo-ku, Kyoto 606, Japan

Address requests for reprints to: Dr. Yoshiyuki Tanaka, Department of Pediatrics, Osaka University Hospital, Osaka University School of Medicine, Fukushima-Ku, Osaka 553, Japan.

Abstract

In order to clarify the role of vitamin D (D) in regulating insulin secretion, we studied the effect of long term (10 days) and short term (3 days) supplementation with D and/or calcium (Ca) on insulin secretion from the isolated, perfused pancreas of D- and Ca-deficient rats. The influence of the nutritional state induced by D deficiency was also evaluated. The long term supplementation of either D, Ca, or both restored the body weight and improved insulin secretion induced by high glucose concentration to the same extent; thus, no significant difference in insulin secretion was found between the D-onlysupplemented group and the Ca-only-supplemented group. When the insulin secretion was compared in D-deplete vs. D-replete rats given the same amount of Ca, insulin secretion was significantly higher in D-replete animals, although plasma Ca levels were also higher. In short term experiments, insulin release was significantly augmented to a similar extent in D- or Ca-replete rats as compared with D- and Ca-deficient rats, despite no significant change in body weight. In a separate experiment, the pancreas from D-deficient rats was perfused with or without 1,25-dihydroxyvitamin D₃ [1,25-OH)₂D₃] to observe its acute effect on insulin release. The perfusion with 1,25-(OH)₂D₃ did not affect insulin release. This result suggests that impaired insulin secretion in D-deficient rats is caused by a decrease in Ca in the body fluid and possibly by the lack of D effect on the pancreas. (Endocrinology 118: 1971–1976, 1986)

Received July 23, 1984.

This article has been cited by other articles:

				R. Bouillon, G. Carmeliet, L. Verlinden, E. van Etten, A. Verstuyf, H. F. Luderer, L. Lieben, C. Mathieu, and M. Demay Vitamin D and Human Health: Lessons from Vitamin D Receptor Null Mice Endocr. Rev., October 1, 2008; 29(6): 726 - 776. [Abstract] [Full Text] [PDF]

				M. B. Zemel Regulation of Adiposity and Obesity Risk By Dietary Calcium: Mechanisms and Implications J. Am. Coll. Nutr., April 1, 2002; 21(2): 146S - 151. [Abstract] [Full Text] [PDF]

				M. Kajikawa, H. Ishida, S. Fujimoto, E. Mukai, M. Nishimura, J. Fujita, Y. Tsuura, Y. Okamoto, A. W. Norman, and Y. Seino An Insulinotropic Effect of Vitamin D Analog with Increasing Intracellular Ca2+ Concentration in Pancreatic {beta}-Cells through Nongenomic Signal Transduction Endocrinology, October 1, 1999; 140(10): 4706 - 4712. [Abstract] [Full Text]