Endocrinology, Vol 119, 1776-1785, Copyright © 1986 by Endocrine Society

ARTICLES

1,25-Dihydroxyvitamin D3 and human bone-derived cells in vitro: effects on alkaline phosphatase, type I collagen and proliferation

JN Beresford, JA Gallagher and RG Russell

1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3], but not 24,25-(OH)2D3 stimulates the alkaline phosphatase activity of cultured human bone cell populations. The stimulatory effect of the sterol was dose dependent (10(-10)-10(-7) M), evident by 24 h, and observed over a range of cell densities. Analysis of the radiolabeled collagens synthesised by human bone cell cultures indicated the synthesis of predominantly type I collagen. In the presence of 1,25-(OH)2D3, but not 24,25-(OH)2D3, there was a dose-dependent (10(-11)-10(-9) M) increase in radiolabeled proline incorporation into collagenase-digestible protein and in the amount of collagen synthesized, expressed as a percentage of the total protein synthesis. The effect of 1,25-(OH)2D3 was observed over a range of cell densities and appeared to be specific for the synthesis of type I collagen. The stimulatory effect of 1,25- (OH)2D3 on alkaline phosphatase activity and the increase in proline incorporation into collagenase-digestible protein were accompanied by a dose-dependent (5 X 10(-11) to 5 X 10(-8) M) inhibition of bone cell proliferation. These findings suggest that 1,25-(OH)2D3 is an important modulator of the growth and differentiation of human bone cells in vitro. They are also consistent with the possibility that 1,25-(OH)2D3 has direct effects on bone formation in vivo.

This article has been cited by other articles:

				Y. Sakaguchi, I. Sekiya, K. Yagishita, S. Ichinose, K. Shinomiya, and T. Muneta Suspended cells from trabecular bone by collagenase digestion become virtually identical to mesenchymal stem cells obtained from marrow aspirates Blood, November 1, 2004; 104(9): 2728 - 2735. [Abstract] [Full Text] [PDF]

				J. H. Davies, B. A. J. Evans, M. E. M. Jenney, and J. W. Gregory Effects of Chemotherapeutic Agents on the Function of Primary Human Osteoblast-Like Cells Derived from Children J. Clin. Endocrinol. Metab., December 1, 2003; 88(12): 6088 - 6097. [Abstract] [Full Text] [PDF]

				R. Tuli, S. Tuli, S. Nandi, M. L. Wang, P. G. Alexander, H. Haleem-Smith, W. J. Hozack, P. A. Manner, K. G. Danielson, and R. S. Tuan Characterization of Multipotential Mesenchymal Progenitor Cells Derived from Human Trabecular Bone Stem Cells, November 1, 2003; 21(6): 681 - 693. [Abstract] [Full Text] [PDF]

				A. Gurlek, M. R. Pittelkow, and R. Kumar Modulation of Growth Factor/Cytokine Synthesis and Signaling by 1{alpha},25-Dihydroxyvitamin D3: Implications in Cell Growth and Differentiation Endocr. Rev., December 1, 2002; 23(6): 763 - 786. [Abstract] [Full Text] [PDF]

				P. A. Zuk, M. Zhu, P. Ashjian, D. A. De Ugarte, J. I. Huang, H. Mizuno, Z. C. Alfonso, J. K. Fraser, P. Benhaim, and M. H. Hedrick Human Adipose Tissue Is a Source of Multipotent Stem Cells Mol. Biol. Cell, December 1, 2002; 13(12): 4279 - 4295. [Abstract] [Full Text] [PDF]

				T. Gaschott, D. Steinhilber, V. Milovic, and J. Stein Tributyrin, a Stable and Rapidly Absorbed Prodrug of Butyric Acid, Enhances Antiproliferative Effects of Dihydroxycholecalciferol in Human Colon Cancer Cells J. Nutr., June 1, 2001; 131(6): 1839 - 1843. [Abstract] [Full Text]

				B. Ecarot and M. Desbarats 1,25-(OH)2D3 Down-Regulates Expression of Phex, a Marker of the Mature Osteoblast Endocrinology, March 1, 1999; 140(3): 1192 - 1199. [Abstract] [Full Text]

				M. E. Nuttall, J. N. Bradbeer, G. B. Stroup, D. P. Nadeau, S. J. Hoffman, H. Zhao, S. Rehm, and M. Gowen Idoxifene: A Novel Selective Estrogen Receptor Modulator Prevents Bone Loss and Lowers Cholesterol Levels in Ovariectomized Rats and Decreases Uterine Weight in Intact Rats Endocrinology, December 1, 1998; 139(12): 5224 - 5234. [Abstract] [Full Text] [PDF]

				S. Jingushi, A. Iwaki, O. Higuchi, Y. Azuma, T. Ohta, J.-I. Shida, T. Izumi, T. Ikenoue, Y. Sugioka, and Y. Iwamoto Serum 1{alpha},25-Dihydroxyvitamin D3 Accumulates into the Fracture Callus during Rat Femoral Fracture Healing Endocrinology, April 1, 1998; 139(4): 1467 - 1473. [Abstract] [Full Text] [PDF]

				B. Komm, C. Terpening, D. Benz, K. Graeme, A Gallegos, M Korc, G. Greene, B. O'Malley, and M. Haussler Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells Science, July 1, 1988; 241(4861): 81 - 84. [Abstract] [PDF]