Endocrinology, Vol 119, 1870-1872, Copyright © 1986 by Endocrine Society

ARTICLES

Carrier-mediated transport of thyroid hormone into rat hepatocytes is rate-limiting in total cellular uptake and metabolism

G Hennemann, EP Krenning, M Polhuys, JA Mol, BF Bernard, TJ Visser and R Docter

We investigated if carrier-mediated transport into rat hepatocytes is rate-limiting in total cellular uptake and metabolism of thyroid hormone. Rat hepatocytes in primary monolayer culture were incubated under equilibrium conditions with tracer T4, T3 or rT3 in the absence or presence of inhibitors of thyroid hormone uptake, i.e., ouabain and ER-22, a monoclonal antibody against the rat hepatocyte plasma membrane. The results for all three iodothyronines show that inhibition of clearance from the medium during incubation is paralleled by a similar decrease in iodide production. This indicates that the decrease in metabolism of thyroid hormone is directly related to the inhibition of cellular uptake. These findings underline the potential importance of the plasma membrane in the regulation of thyroid hormone metabolism and, therefore, determination of expression of thyroid hormone activity.

This article has been cited by other articles:

				R. P. Peeters, E. C. H. Friesema, R. Docter, G. Hennemann, and T. J. Visser Effects of thyroid state on the expression of hepatic thyroid hormone transporters in rats Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1232 - E1238. [Abstract] [Full Text] [PDF]

				G. Hennemann, R. Docter, E. C. H. Friesema, M. de Jong, E. P. Krenning, and T. J. Visser Plasma Membrane Transport of Thyroid Hormones and Its Role in Thyroid Hormone Metabolism and Bioavailability Endocr. Rev., August 1, 2001; 22(4): 451 - 476. [Abstract] [Full Text] [PDF]

				J. W. A. Ritchie, C. J. F. Collingwood, and P. M. Taylor Effect of hypothyroidism on pathways for iodothyronine and tryptophan uptake into rat adipocytes Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E254 - E259. [Abstract] [Full Text] [PDF]

				R. R. Cavalieri, L. A. Simeoni, S. W. Park, J. D. Baxter, B. F. Scharschmidt, R. C. J. Ribeiro, and N. Lomri Thyroid Hormone Export in Rat FRTL-5 Thyroid Cells and Mouse NIH-3T3 Cells Is Carrier-Mediated, Verapamil-Sensitive, and Stereospecific Endocrinology, November 1, 1999; 140(11): 4948 - 4954. [Abstract] [Full Text]

				W. Croteau, J. E. Bodwell, J. M. Richardson, and D. L. St. Germain Conserved Cysteines in the Type 1 Deiodinase Selenoprotein Are Not Essential for Catalytic Activity J. Biol. Chem., September 25, 1998; 273(39): 25230 - 25236. [Abstract] [Full Text] [PDF]

				R. Docter, E. C. H. Friesema, P. G. J. van Stralen, E. P. Krenning, M. E. Everts, T. J. Visser, and G. Hennemann Expression of Rat Liver Cell Membrane Transporters for Thyroid Hormone in Xenopus laevis Oocytes Endocrinology, May 1, 1997; 138(5): 1841 - 1846. [Abstract] [Full Text] [PDF]

				R. C.J. Ribeiro, R. R. Cavalieri, N. Lomri, C. M. Rahmaoui, J. D. Baxter, and B. F. Scharschmidt Thyroid Hormone Export Regulates Cellular Hormone Content and Response J. Biol. Chem., July 19, 1996; 271(29): 17147 - 17151. [Abstract] [Full Text] [PDF]