help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kream, B. E.
Right arrow Articles by Majeska, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kream, B. E.
Right arrow Articles by Majeska, R.

Endocrinology, Vol 119, 1922-1928, Copyright © 1986 by Endocrine Society

ARTICLES

Hormonal regulation of collagen synthesis in a clonal rat osteosarcoma cell line

BE Kream, D Rowe, MD Smith, V Maher and R Majeska

Collagen synthesis in rat osteosarcoma cell line 17/2 (ROS 17/2) was assessed by measuring the incorporation of [3H]proline into collagenase- digestible protein and the formation of [3H]hydroxyproline. PTH and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] inhibited collagen synthesis in ROS 17/2 cells in a time- and dose-dependent manner. PTH reduced collagen synthesis after a 3-h incubation, whereas the effect of 1,25- (OH)2D3 was somewhat slower. Maximal and half-maximal inhibition of collagen synthesis occurred at approximately 1 and 0.1 nM of each hormone, respectively. At confluency, ROS 17/2 cells synthesized 96% type I and 4% type III collagen. PTH reduced the synthesis of type I, but not type III, collagen. PTH and 1,25-(OH)2D3 also reduced procollagen mRNA levels, as determined by a dot blot hybridization assay. Thus, ROS 17/2 cells are a convenient model system for studying the hormonal regulation of collagen metabolism and gene expression in a cloned cell line with the osteoblastic phenotype.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Hussain, F. Wu, C. Christian, and M. J. Kresch
Hyperoxia inhibits fetal rat lung fibroblast proliferation and expression of procollagens
Am J Physiol Lung Cell Mol Physiol, October 1, 1997; 273(4): L726 - L732.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Murakami, A. Nifuji, and M. Noda
Expression of Indian Hedgehog in Osteoblasts and Its Posttranscriptional Regulation by Transforming Growth Factor-{beta}
Endocrinology, May 1, 1997; 138(5): 1972 - 1978.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Dodig, MarkS. Kronenberg, A. Bedalov, BarbaraE. Kream, G. Gronowicz, StephenH. Clark, K. Mack, Y.-H. Liu, R. Maxon, Z. Pan, et al.
Identification of a TAAT-containing Motif Required for High Level Expression of the COL1A1 Promoter in Differentiated Osteoblasts of Transgenic Mice
J. Biol. Chem., July 5, 1996; 271(27): 16422 - 16429.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1986 by The Endocrine Society