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Endocrinology, doi:10.1210/endo-120-2-483
Endocrinology Vol. 120, No. 2 483-490
Copyright © 1987 by the Endocrine Society.
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Partial Purification of a Hypothalamic Factor that Inhibits Gonadotropin-Releasing Hormone-Stimulated Luteinizing Hormone Release*

JONG-CHIN HWAN and MARC E. FREEMAN{dagger}

Department of Biological Science, Florida State University Tallahassee, Florida 32306

Abstract

A protein that suppresses GnRH-stimulated LH release from dispersed rat anterior pituitary cells was purified from rat hypothalami by chromatography on Sephadex G-25, carboxymethyl cellulose, and high performance gel permeation. The final increase in inhibitory activity was 214-fold. The inhibitor is a glycoprotein with an apparent mol wt of 12,252 ± 638, as determined by gel permeation on HPLC and electrophoreses as a monomer after treatment with 8 M urea. The inhibitor has a Stokes radius of 16 A, an S value of 2.14 ± 0.08, an isoelectric point value near 4.1, and a frictional ratio of 1.05. A preliminary amino acid composition is presented. (Endocrinology 120: 483–490,1987)

Footnotes

* This work was supported by NSF Grant PCM-8120408 and the Ford and Mellon II Foundations.

{dagger} To whom all correspondence and requests for reprints should be addressed.

Received December 26, 1985.




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M. Z. Rizwan, R. Porteous, A. E. Herbison, and G. M. Anderson
Cells Expressing RFamide-Related Peptide-1/3, the Mammalian Gonadotropin-Inhibitory Hormone Orthologs, Are Not Hypophysiotropic Neuroendocrine Neurons in the Rat
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Copyright © 1987 by The Endocrine Society