help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kamel, F.
Right arrow Articles by Schneider, V. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kamel, F.
Right arrow Articles by Schneider, V. A.

Endocrinology, Vol 120, 1644-1650, Copyright © 1987 by Endocrine Society

ARTICLES

Effects of luteinizing hormone (LH)-releasing hormone pulse amplitude and frequency on LH secretion by perifused rat anterior pituitary cells

F Kamel, JA Balz, CL Kubajak and VA Schneider

Recent studies have shown that LH secretion in vivo is pulsatile. In the present study, a cell perifusion system was employed to characterize the pituitary response to changes in LHRH pulse amplitude and frequency. Increases in pulse amplitude consistently elevated both mean LH levels and the amount of LH released in response to individual LHRH pulses. The EC50 for LHRH was approximately 3 nM. Increases in pulse frequency also increased mean LH levels, but frequencies of three or more pulses per h were associated with a decrease in the amount of LH released per pulse. Alterations in LHRH pulse characteristics changed qualitative as well as quantitative aspects of LH secretion, with high frequency, high amplitude pulses producing a biphasic response to LHRH. Initially a self-priming response was seen during the second and third hours of stimulation; this was followed by increasing desensitization of the cultures to LHRH. These results, by defining the pituitary response to specific conditions of stimulation, will help to clarify the relationship of LHRH stimulation to LH secretion in vivo.


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
W. R. Duan, J. L. Shin, and J. L. Jameson
Estradiol Suppresses Phosphorylation of Cyclic Adenosine 3',5'-Monophosphate Response Element Binding Protein (CREB) in the Pituitary: Evidence for Indirect Action via Gonadotropin-Releasing Hormone
Mol. Endocrinol., August 1, 1999; 13(8): 1338 - 1352.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Heinze, R. W. Keener, and A. R. Midgley Jr.
A mathematical model of luteinizing hormone release from ovine pituitary cells in perifusion
Am J Physiol Endocrinol Metab, December 1, 1998; 275(6): E1061 - E1071.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. M. Meredith,, F. W. Turek,, and J. E. Levine
Effects of Gonadotropin-Releasing Hormone Pulse Frequency Modulation on the Reproductive Axis of Photoinhibited Male Siberian Hamsters
Biol Reprod, October 1, 1998; 59(4): 813 - 819.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1987 by The Endocrine Society