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Endocrinology, Vol 120, 2357-2366, Copyright © 1987 by Endocrine Society

ARTICLES

Identification of a corticotropin-releasing factor-binding protein in the plasma membrane of AtT-20 mouse pituitary tumor cells and its regulation by dexamethasone

BE Rosendale, DB Jarrett and AG Robinson

CRF stimulates the synthesis and secretion of proopiomelanocortin- derived peptides from AtT-20 mouse pituitary tumor cells. This study has shown that there is a specific binding site for CRF located on the plasma membrane of these cells. Both [125I]iodo-Tyr0CRF and noniodinated CRF (10(-11)-10(-7) M) stimulated, in a dose-dependent manner, the secretion of equimolar amounts of beta-endorphin-like immunoactivity from AtT-20 cells. Disuccinimidyl suberate, a cross- linking agent, was used to demonstrate specific binding of [125I]iodo- Tyr0CRF to plasma membranes from these cells. After cross-linking [125I] iodo-Tyr0CRF, the membrane proteins were solubilized with sodium dodecyl sulfate and electrophoresed on a 10% polyacrylamide gel. A single radioactively labeled band, corresponding to a mol wt of 66,000, was identified by autoradiography. [125I]Iodo-Tyr0CRF binding to these membranes was inhibited by 10(-7) M unlabeled CRF or an equimolar concentration of the CRF analog sauvagine. Similar concentrations (10(- 7) M) of TRH, GnRH, insulin, [Arg8]vasopressin, somatostatin, and ACTH did not inhibit [125I]iodo-Tyr0CRF binding to the plasma membranes. Incubation of AtT-20 cells for 24 h in the presence of 10 nM dexamethasone reduced [125I]iodo-Tyr0CRF binding by 80% compared to that in untreated cells. Dexamethasone also inhibited the CRF- stimulated beta-endorphin-like immunoactivity secretory response. These data indicate that binding of CRF to a specific membrane protein is an integral component in the stimulation of AtT-20 cells by CRF.


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J. L. Green, J. P. Figueroa, G. A. Massmann, J. Schwartz, and J. C. Rose
Corticotropin-Releasing Hormone Type I Receptor Messenger Ribonucleic Acid and Protein Levels in the Ovine Fetal Pituitary: Ontogeny and Effect of Chronic Cortisol Administration
Endocrinology, August 1, 2000; 141(8): 2870 - 2876.
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J.-M. Aubry, A. V. Turnbull, G. Pozzoli, C. Rivier, and W. Vale
Endotoxin Decreases Corticotropin-Releasing Factor Receptor 1 Messenger Ribonucleic Acid Levels in the Rat Pituitary
Endocrinology, April 1, 1997; 138(4): 1621 - 1626.
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Copyright © 1987 by The Endocrine Society