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Endocrinology, Vol 121, 1400-1404, Copyright © 1987 by Endocrine Society

ARTICLES

A morphometric analysis of adrenocortical actin localized by immunoelectron microscopy: the effect of adrenocorticotropin

KE Loesser and S Malamed
Department of Anatomy, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854.

The localization of actin and the effect of ACTH on its concentration was examined in freshly isolated rat adrenocortical cells. Lowicryl K4M- embedded cells were used for the immunoelectron localization of actin; gold was used as a label for immunoreactive sites. Actin was at least 4 times as concentrated at the cortical cytoplasm as in the lipid droplets and at least 5 times as concentrated in the microvilli as in the lipid droplets. ACTH stimulation approximately doubled the concentration of actin in the cortical cytoplasm and increased by 50% the concentration of actin in the microvilli. The microvillar contribution to the cell surface area was 40% higher in ACTH-stimulated cells than it was in unstimulated cells. These results provide quantitative evidence suggesting that actin and the microvilli participate in steroid secretion by the adrenocortical cell.


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M. Cote, M. D. Payet, and N. Gallo-Payet
Association of {alpha}s-Subunit of the Gs Protein with Microfilaments and Microtubules: Implication during Adrenocorticotropin Stimulation in Rat Adrenal Glomerulosa Cells
Endocrinology, January 1, 1997; 138(1): 69 - 78.
[Abstract] [Full Text] [PDF]


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