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Endocrinology, Vol 121, 1577-1579, Copyright © 1987 by Endocrine Society

ARTICLES

Ovarian transforming growth factor-alpha gene expression: immunohistochemical localization to the theca-interstitial cells

JE Kudlow, MS Kobrin, AF Purchio, DR Twardzik, ER Hernandez, SL Asa and EY Adashi
Department of Clinical Biochemistry, University of Toronto, Ontario, Canada.

Rat ovaries were examined for the presence of transforming growth factor-alpha (TGF-alpha). Immature diethylstilbestrol-primed rats were treated for 3 days prior to sacrifice with or without FSH. Their ovaries were either fixed in formaldehyde for immunohistochemistry or the RNA was extracted for northern blot analysis. Hybridization with a rat TGF-alpha cDNA probe revealed a 4.5 kilobase mRNA whose abundance was markedly stimulated in the rats treated with FSH. Immunohistochemical staining, using a sequence specific monoclonal antibody to TGF-alpha, MF9, detected immunoreactive TGF-alpha only in the interstitial and theca cells. FSH treatment resulted in no appreciable change in the immunostaining. These results suggest that TGF-alpha is synthesized in the ovary, perhaps in the theca- interstitial compartment.


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