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Induction of c-fos, Calcitonin Gene Expression, and Acidic Fibroblast Growth Factor Production in a Multipeptide-Secreting Neuroendocrine Cell Line^*

FUSUN N. ZEYTIN, SCOTT F. RUSK, ANDREW BAIRD, V. RAYMOND, S. E. LEFF, TAPIO HAAPARANTA and A. J. MANDELL

Laboratories for Neuroendocrinology and Molecular Biology and Virology Laboratory (V.R.), The Salk Institute, and the Eukaryotic Regulatory Biology Program, School of Medicine (S.E.L.) and the Laboratory of Biological Dynamics and Theoretical Medicine (A.J.M.), University of California La Jolla, California 92037
California Biotechnology, Inc. (T.H.) Mountain View, California 94043

Address requests for reprints to: Dr. Fiisun N. Zeytin, Laboratories for Neuroendocrinology, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037.

Abstract

The multipeptide-secreting 44-2C cell line maintains differentiated function when grown in a serum-free, growth factor- and hormone-deprived milieu. The cells continue to synthesize and secrete calcitonin (CT), CT gene-related peptide, neurotensin, and somatostatin and respond to cellular secretagogues such as GRF and acidic and basic fibroblast growth factor. We designed experiments to ascertain the functional role(s) of cellular factors involved in the maintenance of the differentiated state in 44-2C cells. We report here the phenotypic transformation that occurs in these cells in the course of adjustment to the serum-free state. We also show the differential increase in CT-specific mRNA, the transient induction of c-fos, and the characterization of biologically active acidic fibroblast growth factor (Endocrinology 122: 1114–1120, 1988)

Footnotes

^* This work supported by NIH Grants HD-09690 and DK-18811.

Centennial Fellow of the Medical Research Council of Canada.

Received July 26, 1987.

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