Endocrinology, Vol 122, 1842-1846, Copyright © 1988 by Endocrine Society

ARTICLES

Estrogen suppresses rat gonadotropin gene transcription in vivo

MA Shupnik, SD Gharib and WW Chin
Department of Medicine, Brigham and Women's Hospital, Massachusetts 02115.

The modulation of gonadotropin gene expression in vivo by 17 beta- estradiol (E2) was investigated at the level of gonadotropin subunit gene transcription. Transcription rates were measured in isolated pituitary nuclei from untreated (control), ovariectomized (OVX), or estrogen-replaced (OVX-E2) female Sprague-Dawley rats. In control females, the average rate of alpha-subunit messenger RNA (mRNA) synthesis (161 +/- 30 ppm) was greater than the rates for either LH beta (42 +/- 17 ppm) or FSH beta (57 +/- 21 ppm). In OVX animals the synthesis rate for all three mRNAs was increased (alpha-subunit = 413 +/- 55 ppm; LH beta = 432 +/- 121 ppm; FSH beta = 204 +/- 91 ppm), but the 5- to 10-fold increases noted for LH beta were the most dramatic. Chronic E2 administration (10 micrograms/100 g BW sc daily for 7 days) suppressed these elevated transcription rates to near control values. The E2 effect occurred rapidly, with a significant suppression of all subunit transcription rates at 4 h, and a maximally suppressive effect at 24 h, at which time alpha-subunit = 30%, LH beta = 12%, and FSH beta = 26% of the mRNA synthesis rate in OVX animals. Thus, the estrogen status in vivo can affect gonadotropin subunit gene expression at the transcriptional level. Subunit gene transcription rates are regulated coordinately in a negative fashion, but LH beta mRNA synthesis is affected most dramatically.

This article has been cited by other articles:

				C. Glidewell-Kenney, J. Weiss, L. A. Hurley, J. E. Levine, and J. L. Jameson Estrogen Receptor {alpha} Signaling Pathways Differentially Regulate Gonadotropin Subunit Gene Expression and Serum Follicle-Stimulating Hormone in the Female Mouse Endocrinology, August 1, 2008; 149(8): 4168 - 4176. [Abstract] [Full Text] [PDF]

				T. Kowase, H. E. Walsh, D. S. Darling, and M. A. Shupnik Estrogen Enhances Gonadotropin-Releasing Hormone-Stimulated Transcription of the Luteinizing Hormone Subunit Promoters via Altered Expression of Stimulatory and Suppressive Transcription Factors Endocrinology, December 1, 2007; 148(12): 6083 - 6091. [Abstract] [Full Text] [PDF]

				J. D. Veldhuis, A. Bae, R. S. Swerdloff, A. Iranmanesh, and C. Wang Experimentally Induced Androgen Depletion Accentuates Ethnicity-Related Contrasts in Luteinizing Hormone Secretion in Asian and Caucasian Men J. Clin. Endocrinol. Metab., March 1, 2005; 90(3): 1632 - 1638. [Abstract] [Full Text] [PDF]

				L L Burger, D J Haisenleder, A C Dalkin, and J C Marshall Regulation of gonadotropin subunit gene transcription J. Mol. Endocrinol., December 1, 2004; 33(3): 559 - 584. [Abstract] [Full Text] [PDF]

				J. S. Jorgensen, C. C. Quirk, and J. H. Nilson Multiple and Overlapping Combinatorial Codes Orchestrate Hormonal Responsiveness and Dictate Cell-Specific Expression of the Genes Encoding Luteinizing Hormone Endocr. Rev., August 1, 2004; 25(4): 521 - 542. [Abstract] [Full Text] [PDF]

				J. M. Kuhn, H. Lefebvre, C. Duparc, A. Pellerin, J. P. Luton, and G. Strauch Cosecretion of Estrogen and Inhibin B by a Feminizing Adrenocortical Adenoma: Impact on Gonadotropin Secretion J. Clin. Endocrinol. Metab., May 1, 2002; 87(5): 2367 - 2375. [Abstract] [Full Text] [PDF]

				D. Curtin, S. Jenkins, N. Farmer, A. C. Anderson, D. J. Haisenleder, E. Rissman, E. M. Wilson, and M. A. Shupnik Androgen Suppression of GnRH-Stimulated Rat LH{beta} Gene Transcription Occurs Through Sp1 Sites in the Distal GnRH-Responsive Promoter Region Mol. Endocrinol., November 1, 2001; 15(11): 1906 - 1917. [Abstract] [Full Text] [PDF]

				L. L. Burger, A. C. Dalkin, K. W. Aylor, L. J. Workman, D. J. Haisenleder, and J. C. Marshall Regulation of Gonadotropin Subunit Transcription after Ovariectomy in the Rat: Measurement of Subunit Primary Transcripts Reveals Differential Roles of GnRH and Inhibin Endocrinology, August 1, 2001; 142(8): 3435 - 3442. [Abstract] [Full Text] [PDF]

				A. C. Dalkin, L. L. Burger, K. W. Aylor, D. J. Haisenleder, L. J. Workman, S. Cho, and J. C. Marshall Regulation of Gonadotropin Subunit Gene Transcription by Gonadotropin-Releasing Hormone: Measurement of Primary Transcript Ribonucleic Acids by Quantitative Reverse Transcription-Polymerase Chain Reaction Assays Endocrinology, January 1, 2001; 142(1): 139 - 146. [Abstract] [Full Text] [PDF]

				W. R. Duan, J. L. Shin, and J. L. Jameson Estradiol Suppresses Phosphorylation of Cyclic Adenosine 3',5'-Monophosphate Response Element Binding Protein (CREB) in the Pituitary: Evidence for Indirect Action via Gonadotropin-Releasing Hormone Mol. Endocrinol., August 1, 1999; 13(8): 1338 - 1352. [Abstract] [Full Text]

				S. Kawakami and S. J. Winters Regulation of Lutenizing Hormone Secretion and Subunit Messenger Ribonucleic Acid Expression by Gonadal Steroids in Perifused Pituitary Cells from Male Monkeys and Rats Endocrinology, August 1, 1999; 140(8): 3587 - 3593. [Abstract] [Full Text]

				K. M. Scully, A. S. Gleiberman, J. Lindzey, D. B. Lubahn, K. S. Korach, and M. G. Rosenfeld Role of Estrogen Receptor-{{alpha}} in the Anterior Pituitary Gland Mol. Endocrinol., June 1, 1997; 11(6): 674 - 681. [Abstract] [Full Text]