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Endocrinology, Vol 122, 2127-2133, Copyright © 1988 by Endocrine Society

ARTICLES

Variable effects of dexamethasone on protein synthesis in clonal rat osteosarcoma cells

BO Hodge and BE Kream
Department of Medicine, University of Connecticut Health Center, Farmington 06032.

We examined the effects of dexamethasone on protein synthesis in clonal rat osteoblastic osteosarcoma (ROS) cell lines by measuring the incorporation of [3H]proline into collagenase-digestible and noncollagen protein in the cell layer and medium of the cultures. In ROS 17/2 and subclone C12 of ROS 17/2.8, dexamethasone decreased collagen synthesis with no change in DNA content of the cultures. In ROS 17/2.8 and its subclone G2, dexamethasone stimulated collagen and noncollagen protein synthesis, with a concomitant decrease in the DNA content of the cells. These data indicate that ROS cell lines are phenotypically heterogeneous and suggest that in normal bone there may be distinct subpopulations of osteoblasts with varying phenotypic traits with respect to the regulation of protein synthesis.


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H. W. Woitge, J. R. Harrison, A. Ivkosic, Z. Krozowski, and B. E. Kream
Cloning and in Vitro Characterization of {{alpha}}1(I)-Collagen 11{{beta}}-Hydroxysteroid Dehydrogenase Type 2 Transgenes as Models for Osteoblast-Selective Inactivation of Natural Glucocorticoids
Endocrinology, March 1, 2001; 142(3): 1341 - 1348.
[Abstract] [Full Text] [PDF]


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