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Endocrinology, Vol 122, 2174-2182, Copyright © 1988 by Endocrine Society

ARTICLES

Effect of vitamin D status on chick kidney proteins: detection of a 45- kilodalton mitochondrial protein suppressed by vitamin D

SR Kain, KS Kamrath and HL Henry
Department of Biochemistry, University of California, Riverside 92521.

Two-dimensional polyacrylamide gel electrophoresis along with L- [35S]methionine radiolabeling studies were used to examine the effect of chronic vitamin D status on the composition and relative abundance of chick kidney proteins. Comparison of silver-stained gels revealed no extensive differences in either the electrophoretic mobility or the amounts of kidney proteins present in the mitochondrial fraction from vitamin D-replete and vitamin D-deficient chicks. A similar result was obtained in studies with L-[35S]methionine-labeled proteins. Vitamin D deficiency specifically elevated levels of a 45-kilodalton mitochondrial protein (pI 5.0 to 5.5) by approximately 5- to 12-fold relative to amounts present in vitamin D-replete tissue. This protein could not be detected in postmitochondrial supernatant fractions and was only faintly visible in crude kidney homogenates. The specificity of the observed suppression of this 45-kilodalton protein by vitamin D suggests that it may play an important role in renal functions influenced by the vitamin D endocrine system.




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Copyright © 1988 by The Endocrine Society