help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sakai, T.
Right arrow Articles by Kurosumi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakai, T.
Right arrow Articles by Kurosumi, K.

Endocrinology, Vol 122, 2803-2808, Copyright © 1988 by Endocrine Society

ARTICLES

Effect of passive immunization to gonadotropin-releasing hormone (GnRH) using GnRH antiserum on the mitotic activity of gonadotrophs in castrated male rats

T Sakai, K Inoue, Y Hasegawa and K Kurosumi
Department of Morphology, Gunma University, Maebashi, Japan.

In order to reveal the mechanism of elevation in the mitotic activity of gonadotrophs in the pituitary gland of castrated rats, passive immunization to GnRH designed to block the activity of GnRH in castrates was performed, and changes in the mitotic activity of pituitary gonadotrophs and mammotrophs were studied. The increased serum levels of gonadotropins and their subunits in castrates were dramatically suppressed by the administration of rabbit anti-GnRH serum (RAGnRH). However, this treatment had no effect on the serum levels of PRL, suggesting that the passive immunization to GnRH used in this study was only effective in blocking the hormonal activity of GnRH to the gonadotropin secretion. Mitosis of the gonadotrophs in normal rat anterior pituitary was rarely observed, but it was dramatically increased by castration. This elevated mitotic activity of gonadotrophs in the castrated rats was significantly suppressed by the administration of RAGnRH. On the other hand, mitotic activity of mammotrophs was decreased by castration. This diminution in the mitotic activity of mammotrophs was not changed by administration of RAGnRH. These results showed that GnRH is an important factor for stimulation of gonadotroph cell proliferation in castrated rats.


This article has been cited by other articles:


Home page
 J EndocrinolHome page
L A Nolan and A Levy
The effects of testosterone and oestrogen on gonadectomised and intact male rat anterior pituitary mitotic and apoptotic activity.
J. Endocrinol., March 1, 2006; 188(3): 387 - 396.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Hauspie, E. Seuntjens, H. Vankelecom, and C. Denef
Stimulation of Combinatorial Expression of Prolactin and Glycoprotein Hormone {alpha}-Subunit Genes by Gonadotropin-Releasing Hormone and Estradiol-17{beta} in Single Rat Pituitary Cells during Aggregate Cell Culture
Endocrinology, January 1, 2003; 144(1): 388 - 399.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. A. Tobin, R. P. Millar, and B. J. Canny
Testosterone Acts Directly at the Pituitary to Regulate Gonadotropin-Releasing Hormone-Induced Calcium Signals in Male Rat Gonadotropes
Endocrinology, August 1, 1997; 138(8): 3314 - 3319.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1988 by The Endocrine Society