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Endocrinology, Vol 123, 463-468, Copyright © 1988 by Endocrine Society

ARTICLES

Growth factors regulate immunoreactive insulin-like growth factor-I production by cultured porcine granulosa cells

JS Mondschein and JM Hammond
Department of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.

The effects of various growth factors on the production of immunoreactive insulin-like growth factor I (iIGF-I) in short term (3- day) cultures of porcine granulosa cells was investigated. Epidermal growth factor (EGF) was shown to be a potent dose-dependent stimulator of iIGF-I production, achieving a 3.6-fold stimulation at a dose of 10 ng/ml. Transforming growth factor-alpha (10 ng EGF equivalents/ml) was also stimulatory. Platelet-derived growth factor (10 ng/ml) had no effect of its own, but enhanced EGF-stimulated iIGF-I production. The acidic and basic fibroblast growth factors (100 ng/ml) had no effect alone or in combination with EGF. Transforming growth factor-beta (10 ng/ml) had no effect of its own, but inhibited EGF-stimulated iIGF-I production. The interactive effects of EGF and FSH (200 ng/ml) on iIGF- I production were investigated in short term and longer term (7-day) cultures. In short term cultures under conditions optimized for EGF- dependent iIGF-I production, FSH had no effect of its own and inhibited EGF action. Conversely, in longer term cultures optimized for FSH- dependent iIGF-I production, EGF had no effect of its own and inhibited FSH action. Thus IGF production by cultured porcine granulosa cells is regulated in a complex manner and is highly dependent on the culture conditions. Our results suggest that IGF production in the ovary may also be regulated in a complex manner which is dependent on the developmental state of the follicle.


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