help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sinha, Y. N.
Right arrow Articles by Jacobsen, B. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sinha, Y. N.
Right arrow Articles by Jacobsen, B. P.

Endocrinology, Vol 123, 1364-1370, Copyright © 1988 by Endocrine Society

ARTICLES

Structural and immunologic evidence for a small molecular weight ("21K") variant of prolactin

YN Sinha and BP Jacobsen
Lutcher Brown Department of Biochemistry, Whittier Institute for Diabetes and Endocrinology, Scripps Memorial Hospital, La Jolla, California 92037.

The mol wt (Mr) of intact murine PRL is approximately 23,000. Immunoblotting analysis shows a 21,000 Mr band in fresh rat and mouse pituitary extracts that cross-reacts strongly with PRL antibodies. The band becomes markedly altered by stimuli known to influence PRL secretion, such as nursing, estradiol benzoate, and perphenazine. By two-dimensional electrophoresis, it splits into at least three components, two of which have more acidic pI than PRL. The tyrosine peptide maps of the three proteins resemble that of PRL. These results indicate that the 21,000 Mr band consists of a cluster of proteins that are structural variants of PRL, raising the possibility that these variants result from alternative splicing of the PRL gene transcript.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1988 by The Endocrine Society