help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Calkins, J. H.
Right arrow Articles by Lin, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Calkins, J. H.
Right arrow Articles by Lin, T.

Endocrinology, Vol 123, 1605-1610, Copyright © 1988 by Endocrine Society

ARTICLES

Interleukin-1 inhibits Leydig cell steroidogenesis in primary culture

JH Calkins, MM Sigel, HR Nankin and T Lin
Medical Service, W.J.B. Dorn Veterans Hospital, Columbia, South Carolina 29201.

Inflammation and infection induce an acute phase response. The response is characterized by fever and production of interleukin-1 (IL-1). In the present study we evaluated the effects of interleukin-1 on Leydig cell function in primary culture. hCG-stimulated testosterone formation was markedly reduced by IL-1, with an ED50 of 1 U/ml. Basal testosterone production was slightly enhanced in the presence of low concentrations of IL-1, while high concentrations of IL-1 inhibited testosterone formation. Significant inhibition of hCG-stimulated testosterone formation was noted as early as 8 h after the addition of IL-1. IL-1 also inhibited hCG-stimulated cAMP formation, as well as 8- bromo-cAMP- and forskolin-stimulated testosterone synthesis. Furthermore, LH binding to Leydig cells was reduced by human IL-1. The inhibitory effects of IL-1 were reversed only partially by the addition of a cyclooxygenase inhibitor, indomethacin (0.1 mM), even though prostaglandin E2 formation was completely blocked. This indicates that the observed effects of IL-1 are not completely mediated by increased PGE2 formation. The present study suggests that IL-1 is a potent modulator of Leydig cell steroidogenesis. Decreased testosterone formation may modulate the immune response and contribute to the catabolic changes occurring during infection.


This article has been cited by other articles:


Home page
 EndocrinologyHome page
T. Ishikawa and P. L. Morris
A Multistep Kinase-Based Sertoli Cell Autocrine-Amplifying Loop Regulates Prostaglandins, Their Receptors, and Cytokines
Endocrinology, April 1, 2006; 147(4): 1706 - 1716.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Ishikawa, K. Hwang, D. Lazzarino, and P. L. Morris
Sertoli Cell Expression of Steroidogenic Acute Regulatory Protein-Related Lipid Transfer 1 and 5 Domain-Containing Proteins and Sterol Regulatory Element Binding Protein-1 Are Interleukin-1{beta} Regulated by Activation of c-Jun N-Terminal Kinase and Cyclooxygenase-2 and Cytokine Induction
Endocrinology, December 1, 2005; 146(12): 5100 - 5111.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Iranmanesh and J. D. Veldhuis
Combined Inhibition of Types I and II 5 {alpha}-Reductase Selectively Augments the Basal (Nonpulsatile) Mode of Testosterone Secretion in Young Men
J. Clin. Endocrinol. Metab., July 1, 2005; 90(7): 4232 - 4237.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. Colon, K. V. Svechnikov, C. Carlsson-Skwirut, P. Bang, and O. Soder
Stimulation of Steroidogenesis in Immature Rat Leydig Cells Evoked by Interleukin-1{alpha} Is Potentiated by Growth Hormone and Insulin-Like Growth Factors
Endocrinology, January 1, 2005; 146(1): 221 - 230.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Walch and P. L. Morris
Cyclooxygenase 2 Pathway Mediates IL-1{beta} Regulation of IL-1{alpha}, -1{beta}, and IL-6 mRNA Levels in Leydig Cell Progenitors
Endocrinology, September 1, 2002; 143(9): 3276 - 3283.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
A. L. Asirvatham and B. C. Bruot
Cell-Cell Interactions in the Testis of Adjuvant-Induced Arthritic Rat
J Androl, July 1, 2002; 23(4): 491 - 497.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Lazarus, C. J. Munday, N. Eguchi, S. Matsumoto, G. J. Killian, B. K. Kubata, and Y. Urade
Immunohistochemical Localization of Microsomal PGE Synthase-1 and Cyclooxygenases in Male Mouse Reproductive Organs
Endocrinology, June 1, 2002; 143(6): 2410 - 2419.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
N. Dejucq and B. Jegou
Viruses in the Mammalian Male Genital Tract and Their Effects on the Reproductive System
Microbiol. Mol. Biol. Rev., June 1, 2001; 65(2): 208 - 231.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Sultana, K. Svechnikov, G. Weber, and O. Soder
Molecular Cloning and Expression of a Functionally Different Alternative Splice Variant of Prointerleukin-1{{alpha}} from the Rat Testis
Endocrinology, December 1, 2000; 141(12): 4413 - 4418.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. M. Ogilvie, K. Held Hales, M. E. Roberts, D. Buchanan Hales, and C. Rivier
The Inhibitory Effect of Intracerebroventricularly Injected Interleukin 1ß on Testosterone Secretion in the Rat: Role of Steroidogenic Acute Regulatory Protein
Biol Reprod, February 1, 1999; 60(2): 527 - 533.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Hu, S. You, W. Li, D. Wang, M. L. Nagpal, Y. Mi, P. Liang, and T. Lin
Expression and Regulation of Interferon-{gamma}-Inducible Protein 10 Gene in Rat Leydig Cells
Endocrinology, August 1, 1998; 139(8): 3637 - 3645.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Pelegrin, J. C. Devedjian, C. Costa, J. Visa, G. Solanes, A. Pujol, G. Asins, A. Valera, and F. Bosch
Evidence from Transgenic Mice That Interferon-beta May Be Involved in the Onset of Diabetes Mellitus
J. Biol. Chem., May 15, 1998; 273(20): 12332 - 12340.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Lin, J. Hu, D. Wang, and D. M. Stocco
Interferon-{gamma} Inhibits the Steroidogenic Acute Regulatory Protein Messenger Ribonucleic Acid Expression and Protein Levels in Primary Cultures of Rat Leydig Cells
Endocrinology, May 1, 1998; 139(5): 2217 - 2222.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
L. Gnessi, A. Fabbri, and G. Spera
Gonadal Peptides as Mediators of Development and Functional Control of the Testis: An Integrated System with Hormones and Local Environment
Endocr. Rev., August 1, 1997; 18(4): 541 - 609.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Cudicini, H. Kercret, A.-M. Touzalin, F. Ballet, and B. Jegou
Vectorial Production of Interleukin 1 and Interleukin 6 by Rat Sertoli Cells Cultured in a Dual Culture Compartment System
Endocrinology, July 1, 1997; 138(7): 2863 - 2870.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Tatsumi, M. Fujisawa, M. Kanzaki, Y. Okuda, H. Okada, S. Arakawa, and S. Kamidono
Nitric Oxide Production by Cultured Rat Leydig Cells
Endocrinology, March 1, 1997; 138(3): 994 - 998.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. V. Turnbull and C. Rivier
Inhibition of Gonadotropin-Induced Testosterone Secretion by the Intracerebroventricular Injection of Interleukin-1{beta} in the Male Rat
Endocrinology, March 1, 1997; 138(3): 1008 - 1013.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1988 by The Endocrine Society