Endocrinology, Vol 123, 2089-2099, Copyright © 1988 by Endocrine Society

ARTICLES

Receptors for insulin-like growth factors I and II: autoradiographic localization in rat brain and comparison to receptors for insulin

MA Lesniak, JM Hill, W Kiess, M Rojeski, CB Pert and J Roth
Receptor and Hormone Action Section, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892.

Receptors for insulin-like growth factor I (IGF-I) in rat brain were visualized using autoradiography with [125I]IGF-I. The binding of the labeled peptide was competed for fully by high concentrations of unlabeled IGF-I. At intermediate concentrations of unlabeled peptide the binding of [125I]IGF-I was competed for by unlabeled IGF-I more effectively than by IGF-II or insulin, which is typical of receptors for IGF-I. Essentially every brain section shows specific binding of IGF-I, and the pattern of binding of IGF-I to its receptors correlated well with the cytoarchitectonic structures. In parallel studies we showed that [125I]IGF-II was bound to tissue sections of rat brain and that the binding was competed for by an excess of unlabeled IGF-II. However, intermediate concentrations of unlabeled peptides gave inconclusive results. To confirm that the binding of [125I]IGF-II was to IGF-II receptors, we showed that antibodies specific for the IGF-II receptor inhibited the binding of labeled IGF-II. Furthermore, the binding of the antibody to regions of the brain section, visualized by the application of [125I]protein-A, gave patterns indistinguishable from those obtained with [125I]IGF-II alone. Again, the binding was very widely distributed throughout the central nervous system, and the patterns of distribution corresponded well to the underlying neural structures. Densitometric analysis of the receptors enabled us to compare the distribution of IGF-I receptors with that of IGF-II receptors as well as retrospectively with that of insulin receptors.

This article has been cited by other articles:

				C. Hawkes, D. Kabogo, A. Amritraj, and S. Kar Up-Regulation of Cation-Independent Mannose 6-Phosphate Receptor and Endosomal-Lysosomal Markers in Surviving Neurons after 192-IgG-Saporin Administrations into the Adult Rat Brain Am. J. Pathol., October 1, 2006; 169(4): 1140 - 1154. [Abstract] [Full Text] [PDF]

				C. Hawkes, J. H. Jhamandas, K. H. Harris, W. Fu, R. G. MacDonald, and S. Kar Single Transmembrane Domain Insulin-Like Growth Factor-II/Mannose-6-Phosphate Receptor Regulates Central Cholinergic Function by Activating a G-Protein-Sensitive, Protein Kinase C-Dependent Pathway J. Neurosci., January 11, 2006; 26(2): 585 - 596. [Abstract] [Full Text] [PDF]

				S. S. Daftary and A. C. Gore IGF-1 in the Brain as a Regulator of Reproductive Neuroendocrine Function Experimental Biology and Medicine, May 1, 2005; 230(5): 292 - 306. [Abstract] [Full Text] [PDF]

				R. P. Wettemann, C. A. Lents, N. H. Ciccioli, F. J. White, and I. Rubio Nutritional- and suckling-mediated anovulation in beef cows J Anim Sci, February 1, 2003; 81(14_suppl_2): E48 - 59. [Abstract] [Full Text] [PDF]

				L. Desnoyers, R. A. Simonette, R. L. Vandlen, and B. M. Fendly Novel Non-isotopic Method for the Localization of Receptors in Tissue Sections J. Histochem. Cytochem., December 1, 2001; 49(12): 1509 - 1518. [Abstract] [Full Text] [PDF]

				M. A. I. Aberg, N. D. Aberg, H. Hedbacker, J. Oscarsson, and P. S. Eriksson Peripheral Infusion of IGF-I Selectively Induces Neurogenesis in the Adult Rat Hippocampus J. Neurosci., April 15, 2000; 20(8): 2896 - 2903. [Abstract] [Full Text] [PDF]

				S. L. Bradshaw, A. J. D’Ercole, and V. K. M. Han Overexpression of Insulin-Like Growth Factor-Binding Protein-2 in C6 Glioma Cells Results in Conditional Alteration of Cellular Growth Endocrinology, February 1, 1999; 140(2): 575 - 584. [Abstract] [Full Text]

				H. J. Walter, M. Berry, D. J. Hill, S. Cwyfan-Hughes, J. M. P. Holly, and A. Logan Distinct Sites of Insulin-Like Growth Factor (IGF)-II Expression and Localization in Lesioned Rat Brain: Possible Roles of IGF Binding Proteins (IGFBPs) in the Mediation of IGF-II Activity Endocrinology, January 1, 1999; 140(1): 520 - 532. [Abstract] [Full Text]

				A. Kyttala, O. Heinonen, L. Peltonen, and A. Jalanko Expression and Endocytosis of Lysosomal Aspartylglucosaminidase in Mouse Primary Neurons J. Neurosci., October 1, 1998; 18(19): 7750 - 7756. [Abstract] [Full Text] [PDF]

				C. M. Cheng, G. Joncas, R. R. Reinhardt, R. Farrer, R. Quarles, J. Janssen, M. P. McDonald, J. N. Crawley, L. Powell-Braxton, and C. A. Bondy Biochemical and Morphometric Analyses Show that Myelination in the Insulin-like Growth Factor 1 Null Brain Is Proportionate to Its Neuronal Composition J. Neurosci., August 1, 1998; 18(15): 5673 - 5681. [Abstract] [Full Text] [PDF]

				K. M. Longo, Y. Sun, and A. C. Gore Insulin-Like Growth Factor-I Effects on Gonadotropin-Releasing Hormone Biosynthesis in GT1-7 Cells Endocrinology, March 1, 1998; 139(3): 1125 - 1132. [Abstract] [Full Text] [PDF]

				S. Kar, D. Seto, S. Dore, U.-K. Hanisch, and R. Quirion Insulin-like growth factors-I and -II differentially regulate endogenous acetylcholine release from the rat hippocampal formation PNAS, December 9, 1997; 94(25): 14054 - 14059. [Abstract] [Full Text] [PDF]

				H. J. Walter, M. Berry, D. J. Hill, and A. Logan Spatial and Temporal Changes in the Insulin-Like Growth Factor (IGF) Axis Indicate Autocrine/Paracrine Actions of IGF-I within Wounds of the Rat Brain Endocrinology, July 1, 1997; 138(7): 3024 - 3034. [Abstract] [Full Text] [PDF]

				C. M. Cheng, R. R. Reinhardt, W.-H. Lee, G. Joncas, S. C. Patel, and C. A. Bondy Insulin-like growth factor 1 regulates developing brain glucose metabolism PNAS, August 29, 2000; 97(18): 10236 - 10241. [Abstract] [Full Text] [PDF]