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Anatomical Localization of the Effects of 17β-Estradiol on Oxytocin Receptor Binding in the Ventromedial Hypothalamic Nucleus^*

Department of Neuroendocrinology, Rockefeller University (A.E.J., H.C., B.S.M.) New York, New York 10021
Field Research Center, Rockefeller University (G.F.B.) Millbrook, New York 125

Address all correspondence and requests for reprints to: Dr. Allan E. Johnson, Section on Comparative Studies of Brain and Behavior, Laboratory of Clinical Sciences, National Institute of Mental Health, P.O. Box 289, Poolesville, Maryland 20837.

Abstract

Oxytocin (OT) neurotransmission plays a role in the facilitation of steroid-dependent sexual receptivity in the rat. One way in which the ovarian steroid 17β-estradiol (E₂) has been shown to modulate OT transmission is by increasing OT receptor binding in certain brain areas involved in the regulation of female sexual behavior such as the ventromedial hypothalamic nucleus (VMN). This study was undertaken to describe the distribution of OT receptors within the VMN that are regulated by physiological levels of E₂. With quantitative autoradiographic methods, we measured [³H]OT binding in ovariectomized female rats implanted with Silastic capsules containing cholesterol, 5% E₂ or 100% E₂. In addition, plasma E₂ levels, pituitary progestin receptor binding, and uterine weights were measured in animals from each treatment group. Results of this study showed that physiological levels of E₂ increased [³H]0T binding in caudal regions of the ventrolateral VMN and stimulated maximal uterine growth and pituitary progestin receptor binding. However, in more rostral VMN sections, E₂ induced a dose-dependent increase in [³H]OT binding. These data suggest that ovarian steroids sensitize the brain to OT by increasing OT receptor binding in certain brain areas involved in the regulation of sexual receptivity. (Endocrinology 124: 207–211, 1989)

Footnotes

^* This work was supported by Grant NS-07911 to (A.E.J.), Grant TW-03617 and a fellowship from the Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina (to H.C.), Grant NS-07080 (to B.S.M.), and in part by BRSG-S07RR07065 awarded by the Biomedical Research Support Grant Program, Division of Research Resources, NIH.

Received August 10, 1988.

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