This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by CAMPBELL, D. J. Articles by HABENER, J. F. Search for Related Content PubMed PubMed Citation Articles by CAMPBELL, D. J. Articles by HABENER, J. F.

Hybridization in Situ Studies of Angiotensinogen Gene Expression in Rat Adrenal and Lung^*

Laboratory of Molecular Endocrinology (D.J.C., J.F.H.) and Howard Hughes Medical Institute (J.F.H.), Massachusetts General Hospital and Harvard Medical School Boston, Massachusetts 02114
St.Vincent's Institute of Medical Research (D.J.C.) Fitzroy 3065, Melbourne, Australia

Address all correspondence and requests for reprints to: Dr. D. J. Campbell, St. Vincent's Institute of Medical Research, 41 Victoria Parade, Fitzroy 3065, Melbourne, Australia.

Abstract

Both rat adrenal and lung contain low levels of angiotensinogen mRNA, as shown by Northern blot and nuclease Si analyses of RNA extracted from these tissues. We sought to identify the cellular localization of angiotensinogen mRNA in these two tissues using hybridization in situ of tissues obtained from both control rats and rats administered a combination of dexamethasone, ethynylestradiol, and T₃. For the adrenal of hormone-treated rats, angiotensinogen mRNA was identified in periadrenal fibroblast-like cells and brown adipose tissue. For control rats, positive hybridization was obtained for fibroblast-like cells immediately adjacent to the adrenal capsule, but not for periadrenal brown adipose tissue. No hybridization was obtained for cells of the adrenal cortex, medulla, capsule or vessels. For the lung of hormone treated, but not control rats, angiotensinogen mRNA was identified in perivascular and peribronchial fibroblast-like cells and brown adipose tissue in the lung hilum. No hybridization was obtained for pulmonary parenchyma, bronchi, or vessels. These results confirm the widespread tissue distribution of angiotensinogen mRNA, and provide further evidence for the formation of angiotensin within tissues by mechanisms independent of the circulating reninangiotensin system. (Endocrinology 124: 218–222, 1989)

Footnotes

^* These studies were supported in part by USPHS Grant AM-30834 and by a C. J. Martin Fellowship from the National Health and Medical Research Council of Australia (to D.J.C.).

Received June 27, 1988.

This article has been cited by other articles:

				A. Veerappan, A. C. Reid, R. Estephan, N. O'Connor, M. Thadani-Mulero, M. Salazar-Rodriguez, R. Levi, and R. B. Silver Mast cell renin and a local renin-angiotensin system in the airway: Role in bronchoconstriction PNAS, January 29, 2008; 105(4): 1315 - 1320. [Abstract] [Full Text] [PDF]

				J.-S. Jerng, Y.-C. Hsu, H.-D. Wu, H.-Z. Pan, H.-C. Wang, C.-T. Shun, C.-J. Yu, and P.-C. Yang Role of the renin-angiotensin system in ventilator-induced lung injury: an in vivo study in a rat model Thorax, June 1, 2007; 62(6): 527 - 535. [Abstract] [Full Text] [PDF]

				R. P. Marshall, P. Gohlke, R. C. Chambers, D. C. Howell, S. E. Bottoms, T. Unger, R. J. McAnulty, and G. J. Laurent Angiotensin II and the fibroproliferative response to acute lung injury Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L156 - L164. [Abstract] [Full Text] [PDF]

				R. P. MARSHALL, R. J. MCANULTY, and G. J. LAURENT Angiotensin II Is Mitogenic for Human Lung Fibroblasts via Activation of the Type 1 Receptor Am. J. Respir. Crit. Care Med., June 1, 2000; 161(6): 1999 - 2004. [Abstract] [Full Text]

				M. Ehrhart-Bornstein, J. P. Hinson, S. R. Bornstein, W. A. Scherbaum, and G. P. Vinson Intraadrenal Interactions in the Regulation of Adrenocortical Steroidogenesis Endocr. Rev., April 1, 1998; 19(2): 101 - 143. [Abstract] [Full Text]