help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schneider, A. B.
Right arrow Articles by Dudlak, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schneider, A. B.
Right arrow Articles by Dudlak, D.

Endocrinology, Vol 124, 356-362, Copyright © 1989 by Endocrine Society

ARTICLES

Differential incorporation of sulfate into the chondroitin chain and complex carbohydrate chains of human thyroglobulin: studies in normal and neoplastic thyroid tissue

AB Schneider and D Dudlak
Department of Medicine, Michael Reese Hospital and Medical Center, University of Chicago, Illinois 60616.

Human thyroglobulin (TG) is one of a growing number of glycoproteins that are known to contain sulfate. Among these TG is unusual because it contains sulfate on both its asparagine-linked complex carbohydrate units and its single chondroitin 6-SO4 unit. We incubated tissue fragments prepared from normal and neoplastic thyroid tissue with [35S]sulfate to study the incorporation of sulfate into these two types of carbohydrate acceptor sites. Incubation conditions (0.1 mM sulfate for 16 h) were selected that maintained linear incorporation of [35S]sulfate and retention of more than 90% of iodinated TG in the tissue. Enzyme susceptibility was used to determine incorporation into the complex carbohydrate units (endoglycosidase-F) and the chondroitin 6-SO4 unit (chondroitin ABC lyase). In a representative experiment, 29.8% of the incorporated sulfate was found in the chondroitin 6-SO4 unit during the first hour of incubation. This increased progressively to 72.5% in the chondroitin unit during the incubation period from 8-16 h. A reciprocal decrease occurred in the proportion of sulfate incorporated into the complex carbohydrate units. TG released into the medium and retained in the tissue had the same ratio of sulfate in the two types of carbohydrate units. Neoplastic thyroid tissue incorporated more [35S]sulfate into TG than normal thyroid tissue from the same patient. Neoplastic and normal tissue differed further in the ratios of sulfate incorporated into the two types of carbohydrate units. We conclude that the incorporation of sulfate into the two types of sulfate-containing carbohydrate units of TG does not occur in a fixed ratio and that this differential incorporation of sulfate does not appear to be related to its release from the tissue.


This article has been cited by other articles:


Home page
 J. Clin. Endocrinol. Metab.Home page
P. Kopp, O. K. Arseven, L. Sabacan, T. Kotlar, J. Dupuis, H. Cavaliere, C. L. S. Santos, J. L. Jameson, and G. Medeiros-Neto
Phenocopies for Deafness and Goiter Development in a Large Inbred Brazilian Kindred with Pendred's Syndrome Associated with a Novel Mutation in the PDS Gene
J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 336 - 341.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1989 by The Endocrine Society