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Endocrinology, Vol 124, 363-368, Copyright © 1989 by Endocrine Society

ARTICLES

Inhibitory effect of pure 31-kilodalton bovine inhibin on gonadotropin- releasing hormone (GnRH)-induced up-regulation of GnRH binding sites in cultured rat anterior pituitary cells

QF Wang, PG Farnworth, JK Findlay and HG Burger
Medical Research Centre, Prince Henry's Hospital Campus, Monash Medical Centre, Melbourne, Victoria, Australia.

Primary cultures of enzymatically dispersed rat anterior pituitary cells were used to examine the effect of pure 31 kilodalton bovine inhibin on GnRH-induced up-regulation of GnRH binding sites. After 2 days in culture, the cells were exposed to stimuli with or without test substances for 10 h, followed by evaluation of GnRH binding sites using iodinated GnRH-A (Buserelin) as tracer. Inhibin suppressed GnRH-induced up-regulation of GnRH binding sites in a dose-dependent manner with an IC50 of 0.13 U/ml (5.5 pM). The inhibin-related peptides transforming growth factor-beta, and Mullerian inhibitory substance had no detectable effect (stimulatory or inhibitory), suggesting that the action is specific to inhibin. In addition, inhibin inhibited the calcium ionophore A23187-induced up-regulation of GnRH binding sites, indicating that this effect of inhibin can occur, at least in part, at a stage subsequent to Ca2+ mobilization. Inhibin did not compete with iodinated GnRH-A for GnRH binding sites. In conclusion, pure 31 kilodalton bovine inhibin suppressed GnRH-induced up-regulation of GnRH binding sites in cultured rat anterior pituitary cells, providing direct evidence that inhibin modulates delayed actions of GnRH.


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L. M Bilezikjian, A. L Blount, C. J Donaldson, and W. W Vale
Pituitary actions of ligands of the TGF-{beta} family: activins and inhibins.
Reproduction, August 1, 2006; 132(2): 207 - 215.
[Abstract] [Full Text] [PDF]

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G. V. Childs, B. T. Miller, and W. L. Miller
Differential Effects of Inhibin on Gonadotropin Stores and Gonadotropin-Releasing Hormone Binding to Pituitary Cells from Cycling Female Rats
Endocrinology, April 1, 1997; 138(4): 1577 - 1584.
[Abstract] [Full Text] [PDF]


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