This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by DOUEN, A. G. Articles by HOLLOSZY, J. O. Search for Related Content PubMed PubMed Citation Articles by DOUEN, A. G. Articles by HOLLOSZY, J. O.

Exercise-Induced Increase in Glucose Transporters in Plasma Membranes of Rat Skeletal Muscle^*

ANDRE G. DOUEN, TOOLSIE RAMLAL, AMIRA KLIP, DOUGLAS A. YOUNG, GREGORY D. CARTEE and JOHN O. HOLLOSZY

Division of Cell Biology, Hospital for Sick Children (A.G.D., T.R., A.K.) Toronto, Ontario, M5G 1X8 Canada
The Department of Internal Medicine, Washington University School of Medicine (D.A. Y., G.D.C., J.O.H.) St. Louis, Missouri 63110

Address all correspondence and requests for reprints to: Amira Klip, Ph.D., Hospital for Sick Children, 555 University Avenue, Toronto, M5G 1X8 Canada.

Abstract

A previously developed technique for the isolation of plasma and intracellular membrane fractions from rat skeletal muscle was used to investigate transporter migration after insulin treatment or a bout of exercise (45 min of treadmill). Glucose-inhibitable cytochalasin-B binding was used to estimate the number of glucose transporters. Insulin and exercise caused increases in glucose uptake into the hindlimb muscles of 5- and 3-fold, respectively. Each stimulus also caused a 2-fold increase in the number of glucose transporters in plasma membranes prepared from hindlimb muscles. The insulin-induced increase in plasma membrane transporters was accompanied by a concomitant decrease in transporters from the intracellular pool. In contrast to insulin, there was no concomitant decrease in the number of cytochalasin-B-binding sites in the intracellular membrane fraction from exercised muscles. The ability of both insulin and exercise to increase the number of transporters in the plasma membrane is in accordance with recruitment of transporters as one cause of increased transport activity. However, the inability of exercise to decrease the number of transporters in the insulin-sensitive intracellular pool suggests the existence of either a second recruitable transporter pool or masked glucose transporters in the plasma membrane that are unmasked by the muscle contractile activity. (Endocrinology 124: 449–454, 1989)

Footnotes

^* This work was supported by a grant from the Medical Research Council (to A.K.), and Research Grant DK-18986 and Institutional National Research Service Award AG-00078 from the NIH (to J.O.H.).

Recipient of a Hugh Sellers Award from the Banting and Best Diabetes Centre, Canada.

Recipient of a Medical Research Council Scientist Award.

Received July 27, 1988.

This article has been cited by other articles:

				T. H. Marwick, M. D. Hordern, T. Miller, D. A. Chyun, A. G. Bertoni, R. S. Blumenthal, G. Philippides, A. Rocchini, and on behalf of the American Heart Association Exerci Exercise Training for Type 2 Diabetes Mellitus: Impact on Cardiovascular Risk: A Scientific Statement From the American Heart Association Circulation, June 30, 2009; 119(25): 3244 - 3262. [Full Text] [PDF]

				A. S. Thomas, L. F. Greene, J. D. Ard, R. A. Oster, B. E. Darnell, and B. A. Gower Physical Activity May Facilitate Diabetes Prevention in Adolescents Diabetes Care, January 1, 2009; 32(1): 9 - 13. [Abstract] [Full Text] [PDF]

				W. Derave, N. Straumann, R. A. Olek, and P. Hespel Electrolysis stimulates creatine transport and transporter cell surface expression in incubated mouse skeletal muscle: potential role of ROS Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1250 - E1257. [Abstract] [Full Text] [PDF]

				A. J. Rose and E. A. Richter Skeletal Muscle Glucose Uptake During Exercise: How is it Regulated? Physiology, August 1, 2005; 20(4): 260 - 270. [Abstract] [Full Text] [PDF]

				P. T. Fueger, H. S. Hess, K. A. Posey, D. P. Bracy, R. R. Pencek, M. J. Charron, and D. H. Wasserman Control of Exercise-stimulated Muscle Glucose Uptake by GLUT4 Is Dependent on Glucose Phosphorylation Capacity in the Conscious Mouse J. Biol. Chem., December 3, 2004; 279(49): 50956 - 50961. [Abstract] [Full Text] [PDF]

				D. Accili Lilly Lecture 2003: The Struggle for Mastery in Insulin Action: From Triumvirate to Republic Diabetes, July 1, 2004; 53(7): 1633 - 1642. [Abstract] [Full Text] [PDF]

				J. O. Holloszy A forty-year memoir of research on the regulation of glucose transport into muscle Am J Physiol Endocrinol Metab, March 1, 2003; 284(3): E453 - E467. [Abstract] [Full Text] [PDF]

				M. G. Clark, M. G. Wallis, E. J. Barrett, M. A. Vincent, S. M. Richards, L. H. Clerk, and S. Rattigan Blood flow and muscle metabolism: a focus on insulin action Am J Physiol Endocrinol Metab, February 1, 2003; 284(2): E241 - E258. [Abstract] [Full Text] [PDF]

				E. Tomas, A. Zorzano, and N. B. Ruderman Exercise Effects on Muscle Insulin Signaling and Action: Exercise and insulin signaling: a historical perspective J Appl Physiol, August 1, 2002; 93(2): 765 - 772. [Abstract] [Full Text] [PDF]

				E. B. Marliss and M. Vranic Intense Exercise Has Unique Effects on Both Insulin Release and Its Roles in Glucoregulation: Implications for Diabetes Diabetes, February 1, 2002; 51(90001): S271 - 283. [Abstract] [Full Text] [PDF]

				E. A Richter, W. Derave, and J. F P Wojtaszewski Glucose, exercise and insulin: emerging concepts J. Physiol., September 1, 2001; 535(2): 313 - 322. [Abstract] [Full Text] [PDF]

				M. Caruso, C. Miele, F. Oriente, A. Maitan, G. Bifulco, F. Andreozzi, G. Condorelli, P. Formisano, and F. Beguinot In L6 Skeletal Muscle Cells, Glucose Induces Cytosolic Translocation of Protein Kinase C-alpha and Trans-activates the Insulin Receptor Kinase J. Biol. Chem., October 1, 1999; 274(40): 28637 - 28644. [Abstract] [Full Text] [PDF]

				W. Derave and P. Hespel Role of adenosine in regulating glucose uptake during contractions and hypoxia in rat skeletal muscle J. Physiol., February 15, 1999; 515(1): 255 - 263. [Abstract] [Full Text] [PDF]

				X.-X. Han and A. Bonen Epinephrine translocates GLUT-4 but inhibits insulin-stimulated glucose transport in rat muscle Am J Physiol Endocrinol Metab, April 1, 1998; 274(4): E700 - E707. [Abstract] [Full Text] [PDF]

				T. Hayashi, J. F. P. Wojtaszewski, and L. J. Goodyear Exercise regulation of glucose transport in skeletal muscle Am J Physiol Endocrinol Metab, December 1, 1997; 273(6): E1039 - E1051. [Abstract] [Full Text] [PDF]

				E Ralston and T Ploug GLUT4 in cultured skeletal myotubes is segregated from the transferrin receptor and stored in vesicles associated with TGN J. Cell Sci., January 12, 1996; 109(13): 2967 - 2978. [Abstract] [PDF]

				L. Coderre, K. V. Kandror, G. Vallega, and P. F. Pilch Identification and Characterization of an Exercise-sensitive Pool of Glucose Transporters in Skeletal Muscle J. Biol. Chem., November 17, 1995; 270(46): 27584 - 27588. [Abstract] [Full Text] [PDF]