Endocrinology, Vol 124, 1088-1090, Copyright © 1989 by Endocrine Society

ARTICLES

Human granulosa cells synthesize low molecular weight insulin-like growth factor-binding protein

AM Suikkari, J Jalkanen, R Koistinen, R Butzow, O Ritvos, T Ranta and M Seppala
Department I of Obstetrics and Gynecology, University Central Hospital, Helsinki, Finland.

Human follicular fluid contains insulin-like growth factor I (IGF-I) and its low molecular weight binding protein (IGF-BP). We studied the synthesis of IGF-BP by the granulosa cells obtained after ovarian hyperstimulation for in vitro fertilization. The granulosa cells were cultured for 72 hours in Ham's F-10 medium supplemented with 10% fetal calf serum (FCS). Samples of the culture medium were collected every 24 hours. The IGF-BP concentration in culture medium increased from 1.2 to 2.1 micrograms/l at 48 h and to 3.3 micrograms/l at 72 h. De novo synthesis of IGF-BP was shown by incorporation of labeled methionine into immunoreactive IGF-BP, as detected by SDS polyacrylamide electrophoresis (PAGE) and fluorography. Our results demonstrate synthesis of IGF-BP in the human ovary.

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