Endocrinology, Vol 124, 1582-1584, Copyright © 1989 by Endocrine Society

ARTICLES

Extracellular calcium modulates vitamin D-dependent calbindin-D28K gene expression in chick kidney cells

TL Clemens, SA McGlade, KP Garrett, GL Craviso and GN Hendy
Regional Bone Center, Helen Hayes Hospital, West Haverstraw, NY.

The effect of extracellular calcium ion (Ca2+) concentration on 1,25- dihydroxyvitamin D3 (1,25-(OH)2D3)-induction of vitamin D-dependent calcium-binding protein (calbindin-D28K) and its mRNA levels was examined in primary chick kidney cells in vitro. When exposed to normal medium Ca2+ (1.0 mM), 1,25-(OH)2D3 increased calbindin-D28K mRNA, as measured by Northern analysis, by 4-10 fold over basal levels by 12 to 24 h after addition of hormone. In the presence of 0.5 mM Ca2+, 1,25- (OH)2D3 induced calbindin-D28K mRNA by only 2 fold, whereas, when cells were exposed to 2 mM Ca2+, the induction was 10-15 fold. This calcium modulation of 1,25-(OH)2D3 induction was also observed at the level of calbindin-D28K protein concentrations as measured by radioimmunoassay. The alterations in medium Ca2+ were not associated with any change in the rate of total RNA or protein synthesis. These studies suggest that both Ca2+ and 1,25-(OH)2D3 participate in the regulation of calbindin- D28K gene expression in the kidney.

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