Endocrinology, Vol 124, 1918-1924, Copyright © 1989 by Endocrine Society

ARTICLES

The insulin-mimetic effect of vanadate is not correlated with insulin receptor tyrosine kinase activity nor phosphorylation in mouse diaphragm in vivo

HV Strout, PP Vicario, R Saperstein and EE Slater
Department of Biochemical Endocrinology, Merck, Sharp, and Dohme Research Laboratories, Rahway, New Jerey 07065.

The in vivo administration of sodium orthovanadate stimulated the incorporation of [14C]glucose into [14C] glycogen, in a dose- and time- dependent manner, in mouse diaphragm. Activation of diaphragm insulin receptor was measured by exogenous tyrosine kinase activity and an antibody that recognizes a conformational change in the receptor beta- subunit upon autophosphorylation. Neither method detected insulin receptor activation by in vivo vanadate administration, suggesting that vanadate's insulin-mimetic effect on mouse diaphragm glycogenesis occurs at a site distal to the insulin receptor.

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