help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thibault, G.
Right arrow Articles by Cantin, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thibault, G.
Right arrow Articles by Cantin, M.

Endocrinology, Vol 124, 3109-3116, Copyright © 1989 by Endocrine Society

ARTICLES

Processing of the atrial natriuretic factor propeptide by atrial cardiocytes as revealed by immunocryoultramicrotomy

G Thibault, H Haile-Meskel, E Wrobel-Konrad, M Ballak, R Garcia, J Genest and M Cantin
Laboratory of Pathobiology, Clinical Research Institute of Montreal, Quebec, Canada.

Antibodies were raised against three fragments of the rat ANF molecule: C-terminal atrial natriuretic factor (ANF)-(101-126), N-terminal ANF- (11-37), and the putative cleavage site of the ANF propeptide, ANF-(94- 103). These antibodies were purified by affinity chromatography and revealed a major band (17K) corresponding to the propeptide by Western blot analysis. Antibodies against ANF-(94-103) were used in a RIA. All of the peptides that possess this region (98-99) were able to displace iodinated pro-ANF from the antibody. However, peptides that have only one part of the fragment, such as ANF-(99-126) or ANF-(1-98), failed to displace pro-ANF; human ANF-(79-98) (100 pmol) demonstrated about 0.01% cross-reactivity. Immunohistochemical studies revealed that all atrial cardiocytes are reactive with the three antibodies. Immunocryoultramicrotomy revealed that the propeptide travels, uncleaved, from the Golgi complex to immature granules and mature secretory granules. These results indicate that cleavage of the ANF propeptide does not occur at these sites.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. H. Langenickel, I. Pagel, J. Buttgereit, K. Tenner, M. Lindner, R. Dietz, R. Willenbrock, and M. Bader
Rat corin gene: molecular cloning and reduced expression in experimental heart failure
Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1516 - H1521.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
J. W. Slot, G. Garruti, S. Martin, V. Oorschot, G. Posthuma, E. W. Kraegen, R. Laybutt, G. Thibault, and D. E. James
Glucose Transporter (GLUT-4) Is Targeted to Secretory Granules in Rat Atrial Cardiomyocytes
J. Cell Biol., June 16, 1997; 137(6): 1243 - 1254.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1989 by The Endocrine Society