help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kukita, T.
Right arrow Articles by Roodman, G. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kukita, T.
Right arrow Articles by Roodman, G. D.

Endocrinology, Vol 125, 630-637, Copyright © 1989 by Endocrine Society

ARTICLES

Development of a monoclonal antibody to osteoclasts formed in vitro which recognizes mononuclear osteoclast precursors in the marrow

T Kukita and GD Roodman
Research Service, Audie L. Murphy Veterans Administration Medical Center, San Antonio, Texas 78284.

Osteoclast precursors have not been well characterized because there are no known markers that can detect them. We have used osteoclast-like cells formed in vitro to develop a panel of specific antibodies that react with mature osteoclasts, osteoclast precursors, and other cells in the osteoclast lineage. Monoclonal antibody Kn22 reacted strongly with osteoclast-like multinucleated cells formed in long term marrow cultures and reacted very strongly with freshly isolated bone-derived baboon osteoclasts. Using immune cell panning, Kn22 enriched precursors for osteoclasts. The majority of multinucleated cells (71%) formed from fresh marrow mononuclear cells adherent to Kn22 strongly reacted with a monoclonal antibody that recognizes mature osteoclasts (23c6) and responded appropriately to calcitonin. In contrast, only 23% of multinucleated cells formed from marrow mononuclear cells that were not bound by Kn22 formed osteoclast-like cells. The majority (77%) of these multinucleated cells did not strongly react with the osteoclast- specific monoclonal antibody 23c6 or respond to calcitonin. Thus, we have developed a panel of monoclonal antibodies that recognize cells in the osteoclast lineage. One of these antibodies, Kn22, is unique in that it identifies an osteoclast precursor. The 50K antigen detected by Kn22 appears to be a membrane protein present on osteoclast precursors and osteoclasts that has not been previously identified.


This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
J. Lorenzo, M. Horowitz, and Y. Choi
Osteoimmunology: Interactions of the Bone and Immune System
Endocr. Rev., June 1, 2008; 29(4): 403 - 440.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. A. Hentunen, S. H. Jackson, H. Chung, S. V. Reddy, J. Lorenzo, S. J. Choi, and G. D. Roodman
Characterization of Immortalized Osteoclast Precursors Developed from Mice Transgenic for Both bcl-XL and Simian Virus 40 Large T Antigen
Endocrinology, July 1, 1999; 140(7): 2954 - 2961.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1989 by The Endocrine Society