Endocrinology, Vol 125, 801-809, Copyright © 1989 by Endocrine Society

ARTICLES

Immunohistochemical analysis of human growth hormone-releasing hormone gene expression in transgenic mice

AK Brar, RL Brinster and LA Frohman
Department of Internal Medicine, University of Cincinnati, College of Medicine, Ohio 45267.

The tissue-specific expression of a fusion gene encoding the mouse metallothionein-1 promoter and the coding region of the human GH- releasing hormone (hGRH) gene was studied in transgenic mice by immunohistochemistry using an anti-hGRH serum that does not recognize endogenous mouse GRH. hGRH immunoreactivity (GRH-IR) was detected in specific cells of the pituitary, pancreas, kidney, duodenum, lung, testis, ovary, adrenal, heart, and brain. In the pituitary, using double immunofluorescent staining, GRH-IR was found in some, but not all, somatotrophs, gonadotrophs, thyrotrophs, and mammotrophs. GRH-IR was found in both pancreatic exocrine cells and endocrine islets. Within the islet, GRH-IR was colocalized in A and D cells with glucagon and somatostatin, respectively. Immunopositive cells in other tissues were localized in kidney proximal convoluted tubules, duodenal submucosal glands of Brunner, the smooth muscles of pulmonary arterioles, testicular Leydig cells, oocytes, adrenal medullary chromaffin cells, and cardiac atria. In the brain, GRH-IR was seen in the external layer of the median eminence and in perikarya and fibers of the hypothalamic arcuate nucleus, the parvocellular region of the paraventricular nucleus, the supraoptic nucleus, and the amygdala. Somatostatin-immunoreactive cell bodies and fibers in transgenic and control mouse hypothalamus were not appreciably different. In summary, hGRH expression in transgenic mice occurs in a cell-specific manner in the hypothalamus as well as in numerous other tissues, many of which have secretory functions.

This article has been cited by other articles:

				Y.-H. Huang, A. Ito, and R. Arai Immunohistochemical Localization of Monoamine Oxidase Type B in Pancreatic Islets of the Rat J. Histochem. Cytochem., September 1, 2005; 53(9): 1149 - 1158. [Abstract] [Full Text] [PDF]

				S. Jirawatnotai, A. Aziyu, E. C. Osmundson, D. S. Moons, X. Zou, R. D. Kineman, and H. Kiyokawa Cdk4 Is Indispensable for Postnatal Proliferation of the Anterior Pituitary J. Biol. Chem., December 3, 2004; 279(49): 51100 - 51106. [Abstract] [Full Text] [PDF]

				R. D. Kineman, L. T. Teixeira, G. V. Amargo, K. T. Coschigano, J. J. Kopchick, and L. A. Frohman The Effect of GHRH on Somatotrope Hyperplasia and Tumor Formation in the Presence and Absence of GH Signaling Endocrinology, September 1, 2001; 142(9): 3764 - 3773. [Abstract] [Full Text] [PDF]

				X.-d. Peng, S. Park, M. R. Gadelha, K. T. Coschigano, J. J. Kopchick, L. A. Frohman, and R. D. Kineman The Growth Hormone (GH)-Axis of GH Receptor/Binding Protein Gene-Disrupted and Metallothionein-Human GH-Releasing Hormone Transgenic Mice: Hypothalamic Neuropeptide and Pituitary Receptor Expression in the Absence and Presence of GH Feedback Endocrinology, March 1, 2001; 142(3): 1117 - 1123. [Abstract] [Full Text] [PDF]

				M. Kovacs, R. D. Kineman, A. V. Schally, M. Zarandi, K. Groot, and L. A. Frohman Effects of Antagonists of Growth Hormone-Releasing Hormone (GHRH) on GH and Insulin-Like Growth Factor I Levels in Transgenic Mice Overexpressing the Human GHRH Gene, an Animal Model of Acromegaly Endocrinology, November 1, 1997; 138(11): 4536 - 4542. [Abstract] [Full Text] [PDF]

				L. Gnessi, A. Fabbri, and G. Spera Gonadal Peptides as Mediators of Development and Functional Control of the Testis: An Integrated System with Hormones and Local Environment Endocr. Rev., August 1, 1997; 18(4): 541 - 609. [Abstract] [Full Text] [PDF]