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Endocrinology, Vol 125, 1430-1437, Copyright © 1989 by Endocrine Society

ARTICLES

Insulin increases the steady state level of alpha-1(I) procollagen mRNA in the osteoblast-rich segment of fetal rat calvaria

RG Craig, DW Rowe, DN Petersen and BE Kream
Department of BioStructure and Function, University of Connecticut Health Center, Farmington 06032.

Insulin at 3-100 nM increased the steady state level of alpha-1(I) procollagen mRNA and stimulated collagen synthesis in the osteoblast- rich segment of central bone from 21-day-old fetal rat calvaria. The increases in the level of procollagen mRNA and the rate of collagen synthesis were observed 18 h after the addition of insulin to the cultures. The removal of insulin from calvaria incubated for 24 h with 3 nM insulin caused collagen synthesis and the level of alpha-1(I) procollagen mRNA to return to control values within 5 h. Adding insulin back to calvaria withdrawn from insulin treatment for 3 h did not rescue the decay in collagen synthesis or the level of alpha-1(I) procollagen mRNA. Insulin increased the steady state levels of alpha- 1(I) procollagen mRNA in the presence of the RNA synthesis inhibitor actinomycin-D. Our data suggest that in fetal rat bone, one mechanism by which insulin increases the steady state level of alpha-1(I) procollagen mRNA may be by altering its stability.




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Copyright © 1989 by The Endocrine Society