Endocrinology, Vol 125, 1575-1580, Copyright © 1989 by Endocrine Society

ARTICLES

Insulin-like growth factor I regulates type I procollagen messenger ribonucleic acid steady state levels in bone of rats

C Schmid, HP Guler, D Rowe and ER Froesch
Department of Medicine (Metabolic Unit), University Hospital, Zurich, Switzerland.

RNA extracted from parietal bones of rats was analyzed for the abundance of type I procollagen mRNA by a dot hybridization assay using specific cDNA probes. Hypophysectomized rats, deficient in IGF I, have 4.1-fold lower steady state levels of mRNA encoding the alpha 1 chain of type I collagen when compared to normal rats of the same weight (120 g). Administration of recombinant human insulin-like growth factor I (IGF I) for 6 days restores bone collagen mRNA levels to normal, and a single sc injection of 100 micrograms IGF I results in a 1.9-fold increase of both alpha 1(I) and alpha 2 (I)chain mRNAs after 8 h. Studies with calvaria cells in primary culture and with a bone-derived cell line, PyMS, yielded similar results: IGF I treatment of these osteoblast-like cells for 24 h increased steady state levels of type I procollagen mRNAs. Thus, IGF I appears to contribute to osteoblast mRNA expression for both chains of type I collagen.

This article has been cited by other articles:

				K.-C. Leung, G. Johannsson, G. M. Leong, and K. K. Y. Ho Estrogen Regulation of Growth Hormone Action Endocr. Rev., October 1, 2004; 25(5): 693 - 721. [Abstract] [Full Text] [PDF]

				A. Colao, D. Ferone, P. Marzullo, and G. Lombardi Systemic Complications of Acromegaly: Epidemiology, Pathogenesis, and Management Endocr. Rev., February 1, 2004; 25(1): 102 - 152. [Abstract] [Full Text] [PDF]

				T Hytinantti, E-M Rutanen, M Turpeinen, R Sorva, and S Andersson Markers of collagen metabolism and insulin-like growth factor binding protein-1 in term infants Arch. Dis. Child. Fetal Neonatal Ed., July 1, 2000; 83(1): 17F - 20. [Abstract] [Full Text]

				T. L. McCarthy, C. Ji, Y. Chen, K. Kim, and M. Centrella Time- and Dose-Related Interactions between Glucocorticoid and Cyclic Adenosine 3',5'-Monophosphate on CCAAT/Enhancer-Binding Protein-Dependent Insulin-Like Growth Factor I Expression by Osteoblasts Endocrinology, January 1, 2000; 141(1): 127 - 137. [Abstract] [Full Text] [PDF]

				T. Kawane and N. Horiuchi Insulin-Like Growth Factor I Suppresses Parathyroid Hormone (PTH)/PTH-Related Protein Receptor Expression via a Mitogen-Activated Protein Kinase Pathway in UMR-106 Osteoblast-Like Cells Endocrinology, February 1, 1999; 140(2): 871 - 879. [Abstract] [Full Text]

				Y. Umayahara, C. Ji, M. Centrella, P. Rotwein, and T. L. McCarthy CCAAT/Enhancer-binding Protein delta  Activates Insulin-like Growth Factor-I Gene Transcription in Osteoblasts. IDENTIFICATION OF A NOVEL CYCLIC AMP SIGNALING PATHWAY IN BONE J. Biol. Chem., December 12, 1997; 272(50): 31793 - 31800. [Abstract] [Full Text] [PDF]

				T. L. McCarthy, C. Ji, H. Shu, S. Casinghino, K. Crothers, P. Rotwein, and M. Centrella 17beta -Estradiol Potently Suppresses cAMP-induced Insulin-like Growth Factor-I Gene Activation in Primary Rat Osteoblast Cultures J. Biol. Chem., July 18, 1997; 272(29): 18132 - 18139. [Abstract] [Full Text] [PDF]

				M. J. Thomas, Y. Umayahara, H. Shu, M. Centrella, P. Rotwein, and T. L. McCarthy Identification of the cAMP Response Element That Controls Transcriptional Activation of the Insulin-like Growth Factor-I Gene by Prostaglandin E2 in Osteoblasts J. Biol. Chem., September 6, 1996; 271(36): 21835 - 21841. [Abstract] [Full Text] [PDF]

				J. Billiard, Y. Umayahara, K. Wiren, M. Centrella, T. L. McCarthy, and P. Rotwein Regulated Nuclear-Cytoplasmic Localization of CCAAT/ Enhancer-binding Protein delta in Osteoblasts J. Biol. Chem., April 27, 2001; 276(18): 15354 - 15361. [Abstract] [Full Text] [PDF]

				J. Billiard, S. S. Grewal, L. Lukaesko, P. J. S. Stork, and P. Rotwein Hormonal Control of Insulin-like Growth Factor I Gene Transcription in Human Osteoblasts. DUAL ACTIONS OF cAMP-DEPENDENT PROTEIN KINASE ON CCAAT/ENHANCER-BINDING PROTEIN delta J. Biol. Chem., August 10, 2001; 276(33): 31238 - 31246. [Abstract] [Full Text] [PDF]