help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Granneman, J. G.
Right arrow Articles by Bannon, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Granneman, J. G.
Right arrow Articles by Bannon, M. J.

Endocrinology, Vol 125, 2328-2334, Copyright © 1989 by Endocrine Society

ARTICLES

Neural control of the alpha-subunit of Gs messenger ribonucleic acid in rat brown adipose tissue

JG Granneman and MJ Bannon
Laboratory of Biochemical Pharmacology, Sinai Research Institute, Detroit, Michigan.

Neural stimulation of rat interscapular brown adipose tissue (IBAT) can greatly modify transmembrane signalling in this tissue. As part of an investigation into the molecular mechanisms of such modulation we have examined the effects of surgical denervation of BAT and cold exposure (a treatment that increases neural stimulation of BAT) on the levels of mRNA encoding for the alpha-subunit of Gs, the GTP-binding protein that regulates adenylate cyclase. The G alpha s mRNA content of BAT per unit RNA was 5-10 times greater than that of lung, liver, or caudate- putamen. Surgical denervation of BAT of warm-adapted rats reduced G alpha s mRNA content by 30% within 8 h postsurgery and by 65% by 72 h. Concurrent infusion of norepinephrine completely prevented the denervation-induced decline in G alpha s mRNA at 72 h. Infusion of the beta-adrenergic agonist isoproterenol also prevented the denervation- induced decline in G alpha s mRNA, but infusion of the alpha 1- adrenergic agonist phenylephrine was without effect. Exposing rats to 4 C for 3 days approximately doubled the level of G alpha s mRNA. Interestingly, surgical denervation did not prevent the cold-induced increase in G alpha s mRNA levels, indicating that this effect is independent of local innervation. Despite reducing G alpha s mRNA levels by about 60%, surgical denervation failed to alter the concentration of G alpha s protein in IBAT membranes of warm-adapted rats, as determined by immunoblotting. Previous work indicates that cold exposure does not increase the concentration of G alpha s in IBAT membranes. Taken with the present results, these data indicate that there is no simple relationship between tissue G alpha s mRNA content and membrane G alpha s concentration in IBAT.


This article has been cited by other articles:


Home page
 FASEB J.Home page
S. GSELL, T. ESCHENHAGEN, G. KASPAREIT, M. NOSE, H. SCHOLZ, O. BEHRENS, and T. WIELAND
Apparent up-regulation of stimulatory G-protein {alpha} subunits in the pregnant human myometrium is mimicked by elevated smoothelin expression,
FASEB J, January 1, 2000; 14(1): 17 - 26.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1989 by The Endocrine Society