help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zabala, M. T.
Right arrow Articles by Garcia-Ruiz, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zabala, M. T.
Right arrow Articles by Garcia-Ruiz, J. P.

Endocrinology, Vol 125, 2587-2593, Copyright © 1989 by Endocrine Society

ARTICLES

Regulation of expression of the messenger ribonucleic acid encoding the cytosolic form of phosphoenolpyruvate carboxykinase in liver and small intestine of lactating rats

MT Zabala and JP Garcia-Ruiz
Departamento de Biologia Molecular, Universidad Autonoma de Madrid, Spain.

The content of P-enolpyruvate carboxykinase enzyme mRNA (mRNA(PEPCK)) in the liver and small intestine changes in a coordinate way at various stages of pregnancy, lactation, and weaning periods of rats. On the ninth day of lactation, the accumulation of mRNA(PEPCK) in the liver and small intestine was 82% and 152%, respectively, compared with that in starved control rats. After 18 h of induced weaning, both tissues presented residual levels of mRNA(PEPCK). However, after 60 h of induced weaning, the accumulation of mRNA(PEPCK) increased to 60% in the liver and 76% in the small intestine compared with that in starved control rats. Adrenalectomy and bromocriptine-induced hypoprolactinemia decreased the content of this mRNA in the liver of lactating rats, while diabetes increased it. PEPCK gene transcription, determined in isolated nuclei, was 2.4-fold higher in the liver of lactating rats than in hypoprolactinemic animals, but 3.7-fold lower than that in starved animals. These results suggest that the mRNA(PEPCK) accumulation observed in the liver of lactating rats must be due to an increase in both transcription rate and messenger stabilization. PRL added to primary culture of hepatocytes obtained from lactating rats caused a dose-dependent increase in the level of mRNA(PEPCK) and mRNAc- myc. Therefore, the high PEPCK activity reported during lactation is due to increased amounts of mRNA(PEPCK), which, in turn, is due to a coordinated response at transcriptional and posttranscriptional levels, where PRL seems to play an important role.


This article has been cited by other articles:


Home page
 ReproductionHome page
R A Picazo, J P Garcia Ruiz, J Santiago Moreno, A Gonzalez de Bulnes, J Munoz, G Silvan, P L Lorenzo, and J C Illera
Cellular localization and changes in expression of prolactin receptor isoforms in sheep ovary throughout the estrous cycle
Reproduction, November 1, 2004; 128(5): 545 - 553.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Campillos, M. A. Garcia, F. Valdivieso, and J. Vazquez
Transcriptional activation by AP-2{alpha} is modulated by the oncogene DEK
Nucleic Acids Res., March 1, 2003; 31(5): 1571 - 1575.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1989 by The Endocrine Society