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Regulation of Prolactin Secretion in the Human BLymphoblastoid Cell Line IM-9-P3 by Dexamethasone but not Other Regulators of Pituitary Prolactin Secretion^*

Institute for Hormone and Fertility Research 2000 Hamburg 54, West Germany
The Department of Physiology, University of Manitoba Winnipeg, Manitoba, R3E 0W3 Canada

Address all correspondence and requests for reprints to: Birgit Gellersen, Institute for Hormone and Fertility Research, Grandweg 64, D-2000 Hamburg 54, West Germany.

Abstract

We examined whether the ectopic production of human(h) PRL by the human B-lymphoblastoid cell line IM-9-P3 is under hormonal control. We demonstrate that PRL secretion in this cell line is regulated by dexamethasone but not by other hormones known to modulate PRL secretion in the pituitary or decidua. Dexamethasone caused a reduction of secretion rates to 30% of control values after 3 day paralleled by a decrease in hPRL mRNA levels, without affecting cell viability. Half-life determinations of hPRL mRNA in control and dexamethasonetreated cells revealed a reduction of t from 16 to 4 h. PRL secreted by IM-9-P3 cells did not control its own secretion in an autocrine loop, nor did it serve as an autocrine growth factor.

Footnotes

^* This work is based in part on the doctoral study by B.G. in the Faculty of Biology, University of Hamburg.

Received June 8, 1989.

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