Endocrinology, Vol 126, 1053-1057, Copyright © 1990 by Endocrine Society

ARTICLES

Advancing age results in reduction of intestinal and bone 1,25- dihydroxyvitamin D receptor

RL Horst, JP Goff and TA Reinhardt
U.S. Department of Agriculture, National Animal Disease Center, Ames, Iowa 50010.

Target tissue 1,25-dihydroxyvitamin D [1,25-(OH)2D] receptor was monitored in adult (15- to 18-month-old) and young (4- to 5-week-old) male Holtzman rats. The concentration of unoccupied receptor (femtomoles per mg protein) was significantly higher in the intestine (666 +/- 19 vs. 162 +/- 43) and bone (61 +/- 3.7 vs. 18 +/- 2.5) of young rats compared to that in adults. The dissociation constant (Kd) of the intestinal receptor, however, remained very similar at both ages (young, 0.24 nM; adult, 0.53 nM). A similar age-related decline in unoccupied intestinal receptor was also observed in Fischer 344 rats and cows. Infusion of young and adult Holtzman rats with about 72 ng/kg BW 1,25-(OH)2D3 resulted in similar changes in the concentrations of plasma 1,25-(OH)2D3 (150-160 pg/ml) in both age groups. The 1,25- (OH)2D3 infusions also resulted in significant up-regulation of unoccupied intestinal receptor (femtomoles per mg protein) from 512 +/- 27 to 780 +/- 61 in the young rats and 68 +/- 9.4 to 194 +/- 15 in the adult rats. Receptor up-regulation by 1,25-(OH)2D3 (change from control) was significantly higher (P less than 0.05) in young rats (268 +/- 51 fmol/mg protein) than in adults (125 +/- 8.2 fmol/mg protein). These data suggest that the differences in receptor number in young and adult rats may be responsible for functional changes in target tissue response to 1,25-(OH)2D3 associated with advancing age.

This article has been cited by other articles:

				M. S. Taylor, K. F. Knowlton, M. L. McGilliard, W. M. Seymour, and J. H. Herbein Blood Mineral, Hormone, and Osteocalcin Responses of Multiparous Jersey Cows to an Oral Dose of 25-Hydroxyvitamin D3 or Vitamin D3 Before Parturition J Dairy Sci, June 1, 2008; 91(6): 2408 - 2416. [Abstract] [Full Text] [PDF]

				L. A. Gilad and B. Schwartz Association of estrogen receptor {beta} with plasma-membrane caveola components: implication in control of vitamin D receptor J. Mol. Endocrinol., June 1, 2007; 38(6): 603 - 618. [Abstract] [Full Text] [PDF]

				E J Lock, R Ornsrud, L Aksnes, F A T Spanings, R Waagbo, and G Flik The vitamin D receptor and its ligand 1{alpha},25-dihydroxyvitamin D3 in Atlantic salmon (Salmo salar) J. Endocrinol., June 1, 2007; 193(3): 459 - 471. [Abstract] [Full Text] [PDF]

				Y. Song and J. C. Fleet Intestinal Resistance to 1,25 Dihydroxyvitamin D in Mice Heterozygous for the Vitamin D Receptor Knockout Allele Endocrinology, March 1, 2007; 148(3): 1396 - 1402. [Abstract] [Full Text] [PDF]

				N. Charoenphandhu, K. Tudpor, K. Thongchote, W. Saengamnart, S. Puntheeranurak, and N. Krishnamra High-calcium diet modulates effects of long-term prolactin exposure on the cortical bone calcium content in ovariectomized rats Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E443 - E452. [Abstract] [Full Text] [PDF]

				M. van Abel, S. Huybers, J. G. J. Hoenderop, A. W. C. M. van der Kemp, J. P. T. M. van Leeuwen, and R. J. M. Bindels Age-dependent alterations in Ca2+ homeostasis: role of TRPV5 and TRPV6 Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1177 - F1183. [Abstract] [Full Text] [PDF]

				L. A. Gilad, T. Bresler, J. Gnainsky, P. Smirnoff, and B. Schwartz Regulation of vitamin D receptor expression via estrogen-induced activation of the ERK 1/2 signaling pathway in colon and breast cancer cells J. Endocrinol., June 1, 2005; 185(3): 577 - 592. [Abstract] [Full Text] [PDF]

				G. Duque, M. Macoritto, N. Dion, L.-G. Ste-Marie, and R. Kremer 1,25(OH)2D3 acts as a bone-forming agent in the hormone-independent senescence-accelerated mouse (SAM-P/6) Am J Physiol Endocrinol Metab, April 1, 2005; 288(4): E723 - E730. [Abstract] [Full Text] [PDF]

				B. Larsson and I. Nemere Effect of Growth and Maturation on Membrane-Initiated Actions of 1,25-Dihydroxyvitamin D3. I. Calcium Transport, Receptor Kinetics, and Signal Transduction in Intestine of Male Chickens Endocrinology, May 1, 2003; 144(5): 1726 - 1735. [Abstract] [Full Text] [PDF]

				J. C. Fleet, F. Eksir, K. W. Hance, and R. J. Wood Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell lines Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G618 - G625. [Abstract] [Full Text] [PDF]

				J. C. Gallagher, S. E. Fowler, J. R. Detter, and S. S. Sherman Combination Treatment with Estrogen and Calcitriol in the Prevention of Age-Related Bone Loss J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3618 - 3628. [Abstract] [Full Text] [PDF]

				S. Pattanaungkul, B. L. Riggs, A. L. Yergey, N. E. Vieira, W. M. O’Fallon, and S. Khosla Relationship of Intestinal Calcium Absorption to 1,25-Dihydroxyvitamin D [1,25(OH)2D] Levels in Young Versus Elderly Women: Evidence for Age-Related Intestinal Resistance to 1,25(OH)2D Action J. Clin. Endocrinol. Metab., November 1, 2000; 85(11): 4023 - 4027. [Abstract] [Full Text]

				H. J. Armbrecht, M. A. Boltz, and V. B. Kumar Intestinal plasma membrane calcium pump protein and its induction by 1,25(OH)2D3 decrease with age Am J Physiol Gastrointest Liver Physiol, July 1, 1999; 277(1): G41 - G47. [Abstract] [Full Text] [PDF]

				T. A Outila, P. H Mattila, V. I Piironen, and C. J. Lamberg-Allardt Bioavailability of vitamin D from wild edible mushrooms (Cantharellus tubaeformis) as measured with a human bioassay Am. J. Clinical Nutrition, January 1, 1999; 69(1): 95 - 98. [Abstract] [Full Text] [PDF]

				Y. Liel, S. Shany, P. Smirnoff, and B. Schwartz Estrogen Increases 1,25-Dihydroxyvitamin D Receptors Expression and Bioresponse in the Rat Duodenal Mucosa Endocrinology, January 1, 1999; 140(1): 280 - 285. [Abstract] [Full Text]

				R. J. Wood, J. C. Fleet, K. Cashman, M. E. Bruns, and H. F. Deluca Intestinal Calcium Absorption in the Aged Rat: Evidence of Intestinal Resistance to 1,25(OH)2 Vitamin D Endocrinology, September 1, 1998; 139(9): 3843 - 3848. [Abstract] [Full Text] [PDF]

				C. Chen and D. N. Kalu Modulation of Intestinal Estrogen Receptor by Ovariectomy, Estrogen and Growth Hormone J. Pharmacol. Exp. Ther., July 1, 1998; 286(1): 328 - 333. [Abstract] [Full Text]