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Inhibition of 5'-Deiodination of Thyroxine Suppresses the Cold-Induced Increase in Brown Adipose Tissue Messenger Ribonucleic Acid for Mitochondrial Uncoupling Protein without Influencing Lipoprotein Lipase Activity^*

RUSSEL J. REITER, SUSANNE KLAUS, CARSTEN EBBINGHAUS, GERHARD HELDMAIER, UWE REDLIN, DANIEL RICQUIER, MARY K. VAUGHAN and STEPHAN STEINLECHNER

Department of Cellular and Structural Biology, University of Texas Health Science Center (R.J.R., M.K. V.) San Antonio, Texas 78284–7762
Centre de Recherches sur la Nutrition, Centre National de la Recherche Scientifique (S.K., D.R.) 92190 Meudon-Bellevue, France
the Department of Biology, Philipps-Uniuersity (C.E., G.H., U.R., S.S.) D-3550 Marburg, West Germany

Address requests for reprints to: Russel J. Reiter, Department of Cellular and Structural Biology, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284-7762.

Abstract

Young adult male and female Djungarian hamsters were exposed to ambient temperatures of 23 or 0 C for 12 h; half of the animals in each group were treated with iopanoic acid to suppress the peripheral conversion of T₄ to the thermotropically active thyroid hormone T₃ by the enzyme 5'-deiodinase (5'D). Brown adipose tissue (BAT) mRNA for uncoupling protein (UCP), BAT lipoprotein lipase (LPL) activity, and 5'D activity were measured at the conclusion of the study. A temperature of 0 C produced large rises in 5'D and LPL activities and a similar large increase in UCP mRNA within the 12-h exposure period. When 5'D activity was inhibited with iopanoic acid, mRNA for UCP was reduced, while LPL activity was unaffected. The results show that the optimal production of mRNA for BAT UCP depends on the availability of T3; however, T3 is not required for the cold-induced activation of LPL activity in BAT. (Endocrinology 126: 2550–2554, 1990)

Footnotes

^* This work was supported by grants from the NSF (DCB-87–11241 to R.J.R.), the Deutsche Forschungsgemeinschaft (SFB to G.H. and S.S.), and the Centre National de la Recherche Scientifique (to D.R.).

Recipient of a Senior U.S. Scientist Award from the Alexander von Humboldt Foundation, West Germany.

Postdoctoral fellow supported by Deutsche Forschungsgemeinschaft.

Received January 11, 1990.

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