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Production of Mouse Placental Lactogen-I by Trophoblast Giant Cells in Utero and in Vitro^*

Department of Anatomy, Wright State University School of Medicine Dayton, Ohio 45435

Address all correspondence and requests for reprints to: Dr. Gary L. Nieder, Department of Anatomy, Wright State University School of Medicine, Dayton, Ohio 45435.

Abstract

Mouse placental lactogen-I (mPL-I) is the earliest appearing member of the PRL-GH family of placental hormones. Using immunological techniques, we have localized mPL-I both in utero and in vitro to trophoblast giant cells. Detectable amounts of mPL-I were first seen by immunohistochemistry on day 6 of gestation in the mural trophoblast. On day 7 giant cells of the ectoplacental cone also contained mPLI, and on day 10 giant cells adjacent to both the decidua basalis and decidua capsularis stained positive. By day 12 however, all intra- and extraembryonic tissues were negative. The pattern of mPL-I staining in the placenta is consistent with its reported gestational serum profile. mPL-I was also present in trophoblast giant cells in vitro shortly after attachment and spreading of day 5 blastocysts. Analysis of conditioned medium from blastocyst outgrowths by immunoprecipitation and two-dimensional electrophoresis, and by immunoblotting, identified mPL-I as the major secretory product from the trophoblast, consisting of a group of acidic proteins of 30,000–45,000 Mr. The synthesis and secretion of mPL-I in vitro occurred in a serum-free medium suggesting that production of this hormone is part of an endogenous program of trophoblast differentiation. (Endocrinology 126: 2809–2814, 1990)

Footnotes

^* This work was supported by NIH Grant HD-25236.

Received December 18, 1989.

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