Endocrinology, Vol 127, 759-765, Copyright © 1990 by Endocrine Society

ARTICLES

Fed, but not fasted, adrenalectomized rats survive the stress of hemorrhage and hypovolemia

DN Darlington, RB Neves, T Ha, G Chew and MF Dallman
Department of Physiology, University of California, San Francisco 94143- 0444.

We have recently shown that conscious adrenalectomized rats exhibit nearly normal recovery of arterial blood pressure during the 5 h after hemorrhage. In those experiments, it appeared that a previous reduction in food intake might have compromised the recovery of blood pressure and increased mortality. These experiments were designed to test in conscious sham-adrenalectomized (control) and adrenalectomized rats prepared with indwelling arterial and venous cannulae 1) the effects of a 20- to 24-h fast (compared to rats fed ab libitum) on the mobilization of plasma substrates and recovery of arterial blood pressure after a 15 ml/kg.5 min hemorrhage, and 2) vascular responsivity to pressor agents in fed or fasted groups before or 2 h after hemorrhage. In all rats hemorrhage resulted in decreased arterial pressure and heart rate. Arterial pressure recovered to near normal in both fed and fasted control groups and in the fed adrenalectomized rats, and all of these rats survived for 24 h after stress. By contrast, in the fasted adrenalectomized rats, arterial pressure recovered only during the first 1.5-2 h and then failed, resulting in 100% mortality by 3-5 h. Compared to the other three groups, in which substrate levels either increased or remained fairly stable, plasma glucose and beta-hydroxybutyrate concentrations fell steadily from 1.5- 2 h after hemorrhage until death occurred in the fasted adrenalectomized rats. Basal ACTH concentrations were elevated compared to control values in both adrenalectomized groups (fed and fasted). Hemorrhage caused increases in plasma ACTH in all groups; the magnitude of the responses did not differ among the groups. The dilution of Evans' blue dye after hemorrhage (used as an index of fluid movement into the vascular space) was not different in control and adrenalectomized rats (either fed or fasted). There were no differences in pressor responses to phenylephrine, vasopressin, or angiotensin-II between the fed and fasted conditions in the control rats either before or after hemorrhage. There was a fasting-associated decrease in vascular responsivity to vasopressin, but normal responsivity to phenylephrine and angiotensin-II, in the adrenalectomized rats both before and after hemorrhage.(ABSTRACT TRUNCATED AT 400 WORDS)

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