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Endocrinology, Vol 127, 2038-2040, Copyright © 1990 by Endocrine Society

ARTICLES

A rapid and simple one step method for isolation of poly(A)+ RNA from cells in monolayer

KK Hartmann, V Papa, EJ Brown, U Doerries, SM Rosenthal and ID Goldfine
Diabetes and Endocrine Research Department, Mount Zion Hospital and Medical Center, San Francisco, CA 94120.

A simple method was developed to isolate low abundance hormone receptor poly(A)+ RNA from cells in tissue culture. Adherent cells in tissue culture plates were directly released with proteinase K and solubilized in SDS. Oligo(dT) cellulose was directly added to the lysate to obtain poly(A)+ RNA. Yields and purity of the poly(A)+ RNA were comparable to other more lengthy methods. IGF-I receptor and insulin receptor mRNA could be detected on Northern blot without any degradation.




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Copyright © 1990 by The Endocrine Society