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Endocrinology, Vol 127, 2517-2522, Copyright © 1990 by Endocrine Society
ARTICLES |
RA Fraser, HM Kronenberg, PK Pang and S Harvey
Department of Physiology, University of Alberta, Edmonton, Canada.
Polyadenylated RNA, extracted from rat hypothalami, cross-hybridized with a RNA probe complementary in sequence to rat PTH (rPTH) messenger RNA (mRNA). Amplification of complementary DNA (cDNA) by the polymerase chain reaction also demonstrated the presence of rPTH mRNA in the rat hypothalamus and parathyroid gland. rPTH mRNA was localized by in situ hybridization in the paraventricular and supraoptic nuclei of the rat hypothalamus. These results demonstrate the expression of the PTH gene in the central nervous system of the rat in areas which suggest roles for PTH in neuroendocrine function.
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