Endocrinology, Vol 127, 3151-3159, Copyright © 1990 by Endocrine Society

ARTICLES

Interleukin-2 driven nuclear translocation of prolactin in cloned T- lymphocytes

CV Clevenger, AL Sillman and MB Prystowsky
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104.

The nuclear translocation of PRL is demonstrated at the immunofluorescence and electron microscopic (EM) levels in interleukin- 2 (IL2)-stimulated cloned T-cells and concanavalin A-stimulated splenocytes. This translocation occurs 2-10 h after IL2 stimulation, and is reversed by the addition of anti-PRL antiserum into the extracellular culture medium. The nuclear localization of PRL in IL2 stimulated T-cells was confirmed by postembedding immunogold EM. The nuclear uptake of PRL after IL2 stimulation was further documented by EM studies using PRL-colloidal gold conjugates. These studies suggest that the intranuclear PRL is translocated from the extracellular medium via an endosomal/lysosomal pathway over a period of several hours. Finally, the requirement for PRL no later than 6 h after IL2 stimulation is demonstrated through the reversible inhibition of T-cell growth with anti-PRL antiserum.

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